Sequence analysis, transcriptional and posttranscriptional regulation of the rice VDAC family

Al Bitar Fawaz, Roosens Nancy, Smeyers Mathias, Vauterin Marc, Van Boxtel Jos, Jacobs Michel, Homblé Fabrice. 2003. Sequence analysis, transcriptional and posttranscriptional regulation of the rice VDAC family. Biochimica and Biophysica Acta, N. Gene structure and Expression, 1625 : pp. 43-51.

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Abstract : The voltage-dependent anion-selective channel (VDAC) is a mitochondrial outer membrane ion channel. Different isoforms exist in plants but information about their specific role remains to be established. Our purpose is to find out the structural features common to three rice VDAC isoforms and to investigate their (post)transcriptional regulation in response to an osmotic stress. Two new cDNAs encoding mitochondrial VDAC from rice (Oryza sativa) were isolated, sequenced and characterized: a phylogenetic reconstruction permitted identification of orthologues in Poaceae and computer-based analyses predicted 18 transmembrane [bêta]-strands, one amphipathic [alpha]-helix and two different phosphorylation motifs. The expression of three rice vdac genes was investigated. Northern blot analyses indicated that they were expressed in all plant tissues. There was a differential expression of osvdacl and osvdac3, whereas osvdac2 was homogeneously expressed in all tissues. No change in vdac expression was observed under an osmotic stress. However, a fast-enhanced expression of vdac was observed in roots during the recovery period after stress release. This enhanced expression is not correlated to the amount of VDAC protein detected in roots suggesting a posttranscriptional regulation. © 2002 Elsevier Science B.V. All rights reserved. © 2002 Elsevier Science B.V. All rights reserved.

Mots-clés Agrovoc : Oryza sativa, Gène, Identification, Expression des gènes, Mitochondrie, Échange d'ion, Séquence nucléotidique, ADN, Phylogénie

Classification Agris : F30 - Plant genetics and breeding

Auteurs et affiliations

  • Al Bitar Fawaz, ULB (BEL)
  • Roosens Nancy, Vrije Universiteit Brussel (BEL)
  • Smeyers Mathias, ULB (BEL)
  • Vauterin Marc, Vrije Universiteit Brussel (BEL)
  • Van Boxtel Jos, CIRAD-AMIS-BIOTROP (FRA)
  • Jacobs Michel, Vrije Universiteit Brussel (BEL)
  • Homblé Fabrice, ULB (BEL)

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