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Inhibition of avian metapneumovirus (AMPV) replication by RNA interference targeting nucleoprotein gene (N) in cultured cells

Ferreira Helena Lage, Spilki Fernando Rosado, Servan de Almeida Renata, Santos Marcia M.A.B., Arns Clarice Weis. 2007. Inhibition of avian metapneumovirus (AMPV) replication by RNA interference targeting nucleoprotein gene (N) in cultured cells. Antiviral Research, 74 : 77-81.

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Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Résumé : Avian metapneumovirus (AMPV) is the primary causative agent of severe rhinotracheitis in turkeys. It is associated with swollen head syndrome in chickens and is the source of significant economic losses to animal food production. In this study, we designed specific short interfering RNA (siRNA) targeting the AMPV nucleoprotein (N) and fusion (F) genes. Three days post-virus infection, virus titration, real time RT-PCR, and RT-PCR assays were performed to verify the effect of siRNA in AMPV replication. A marked decrease in virus titers from transfected CER cells treated with siRNA/N was observed. Also, the production of N, F, and G mRNAs in AMPV was decreased. Results indicate that N-specific siRNA can inhibit virus replication. In future studies, a combination of siRNAs targeting the RNA polymerase complex may be used as a tool to study AMPV replication and/or antiviral therapy. © 2006 Elsevier B.V. All rights reserved.

Classification Agris : L73 - Maladies des animaux

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

  • Ferreira Helena Lage, UNICAMP (BRA)
  • Spilki Fernando Rosado, UNICAMP (BRA)
  • Servan de Almeida Renata, CIRAD-EMVT-UPR Contrôle des maladies (FRA)
  • Santos Marcia M.A.B., UNICAMP (BRA)
  • Arns Clarice Weis, UNICAMP (BRA)

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