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Evidence for multiplication of the leafhopper-borne maize yellow stripe virus in its vector using ELISA and dot-blot hybridization

Ammar El-Desouky, Khlifa Elham, Mahmoud Amal, Abol-Ela S.E., Peterschmitt Michel. 2007. Evidence for multiplication of the leafhopper-borne maize yellow stripe virus in its vector using ELISA and dot-blot hybridization. Archives of Virology, 152 (3) : pp. 489-494.

Journal article ; Article de revue à facteur d'impact
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Abstract : Maize yellow stripe virus (MYSV) has several features in common with tenuiviruses, but is transmitted by a leafhopper, Cicadulina chinai (Cicadellidae, Hemiptera), rather than planthoppers (Delphacidae, Hemiptera). Herein, MYSV was shown to be propagatively transmitted like tenuiviruses. MYSV RNA was not detected in leafhoppers by dot blot hybridization one day following a 2-day acquisition access period (AAP), but was detected in single or groups of leafhoppers 5-20 days post-acquisition. Likewise, capsid protein of MYSV was not detected by ELISA in single leafhoppers until the third day after the beginning of a 1- or 3-day AAP, but subsequently, mean absorbance values (405 nm) increased gradually, reaching their highest levels 8-14 days post-acquisition. The percentage of ELISA-positive leafhoppers also increased during the same period. Unlike most tenuiviruses, transovarial transmission of MYSV was not detected in 600 C. chinai nymphs that hatched from eggs laid by females that had acquired MYSV from diseased plants. The implications of our findings for MYSV classification are discussed. (Résumé d'auteur)

Mots-clés Agrovoc : Géminivirus striure du maïs, Tenuivirus, Cicadulina, Vecteur de maladie, Hybridation, Test ELISA, Transmission des maladies, Classification

Mots-clés complémentaires : Cicadulina chinai

Classification Agris : H10 - Pests of plants
H20 - Plant diseases

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Ammar El-Desouky, Cairo University (EGY)
  • Khlifa Elham, Cairo University (EGY)
  • Mahmoud Amal, Cairo University (EGY)
  • Abol-Ela S.E., Cairo University (EGY)
  • Peterschmitt Michel, CIRAD-AMIS-UMR BGPI (FRA)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/539524/)

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