Detection of Banana mild mosaic virus (BanMMV) and banana virus X (BVX) by PDO RT PCR

Teycheney Pierre-Yves, Acina Isabelle Nina, Lockhart Benham E.L., Candresse Thierry. 2007. Detection of Banana mild mosaic virus (BanMMV) and banana virus X (BVX) by PDO RT PCR. In : First ISHS : ProMusa ProMusa Symposium: Recent advances in banana crop protection for sustainable production and improved livelihoods, White River, South Africa, 10-14 september 2007. ISHS. Montpellier : Cirad, Résumé, 1 p. International Symposium on recent advances in banana crop protection for sustainable production and improved livelihoods. 1, White River, Afrique du Sud, 10 September 2007/14 September 2007.

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Abstract : Viruses are important constraints to the movement and propagation of plant germplasm, especially for vegetatively propagated crops such as banana and plantain. Their control relies primarily on the use of virus-free plant material, whose production and certification requires sensitive and reliable detection methods. Two members of the family Flexiviridae infect Musa spp : Banana mild mosaic virus (BanMMV) and Banana virus X (BVX). BanMMV causes mild leaf chlorosis symptoms in single infection but mixed infections with Cucumber mosaic virus (CMV) or Banana streak virus (BSV) can lead to important leaf necrosis. BanMMV transmission occurs mainly through vegetative propagation although recent data showed that plant-to-plant transmission occurs at a low rate [1]. BanMMV has therefore become an important constraint to the conservation, movement and exchange of Musa germplasm. Banana virus X was recently identified in Guadeloupe [2]. No symptom can be associated to the presence of this virus and no prevalence study could be carried out due to the lack of a detection method. Therefore, an existing polyvalent reverse transcription degenerate oligonucleotide PCR assay (PDO RTPCR, [3]) was adapted to the specific detection of BanMMV and BVX from banana plants [4]. Polyvalent degenerate oligonucleotides (PDO) containing inosine residues were found to be well suited to the detection of BanMMV, despite its high molecular diversity [1], but not to that of BVX, for which species-specific primers were designed. Sampling and sample processing steps were optimized in order to avoid nucleic acid purification prior to the reverse transcription step. A polyclonal anti-BanMMV antiserum was raised and successfully used for the immunocapture (IC) of BanMMV viral particles from leaf extracts, leading to the development of a PDO-IC-RT-nested PCR assay. Although the anti-BanMMV antiserum could recognize to some extent BVX viral particles, direct binding (DB) was shown to be a more efficient method for processing BVX-infected samples and a PDO-DB-RT-nested PCR assay was developed for the detection of BVX from leaf extracts. (Texte intégral)

Mots-clés Agrovoc : Musa, Virus des végétaux, Identification, PCR, Test biologique

Mots-clés géographiques Agrovoc : Guadeloupe

Mots-clés complémentaires : Virus de la mosaïque

Classification Agris : H20 - Plant diseases
F30 - Plant genetics and breeding

Auteurs et affiliations

  • Teycheney Pierre-Yves, CIRAD-BIOS-UPR Multiplication végétative (GLP) ORCID: 0000-0002-9754-0745
  • Acina Isabelle Nina, CIRAD-BIOS-UPR Multiplication végétative (GLP)
  • Lockhart Benham E.L., University of Minnesota (USA)
  • Candresse Thierry, INRA (FRA)

Autres liens de la publication

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