A PCR for the detection of mycoplasmas belonging to the Mycoplasma mycoides cluster : application to the diagnosis of contagious agalactia

Woubit Salah, Manso-Silvan Lucia, Lorenzon Sophie, Gaurivaud Patrice, Poumarat François, Pellet Marie-Pierre, Singh V.P., Thiaucourt François. 2007. A PCR for the detection of mycoplasmas belonging to the Mycoplasma mycoides cluster : application to the diagnosis of contagious agalactia. Molecular and Cellular Probes, 21 : pp. 391-399.

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Abstract : Contagious agalactia is a mycoplasmal infection caused by Mycoplasma agalactiae, Mycoplasma mycoides subsp. mycoides LC, M. mycoides subsp. capri, Mycoplasma capricolum subsp. capricolum and Mycoplasma putrefaciens. Identification of the causative organisms is usually performed by isolation and classical biochemical and serological tests, though this is a lengthy and cumbersome process for mycoplasmas. Specific PCR assays have been developed for the identification of Mycoplasma agalactiae and M. putrefaciens. For members of the M. mycoides cluster existing PCR tests are based on the amplification of highly conserved genes coding for ribosomal proteins, hence a possibility of cross-reactions. The gene glk, coding for a glucokinase, that is found in this cluster is very distantly related to any other bacterial glucokinase described so far. It was therefore chosen as target to design a new PCR test. The validation was performed independently in three laboratories in France and India using over 100 mycoplasma strains of various geographical origins. All strains belonging to the M. mycoides cluster were detected by amplification of the expected PCR product (428 bp) while no amplification was obtained from M. agalactiae strains. Our results demonstrate the universality of this PCR in spite of the great heterogeneity found within this cluster. This new tool may be of great help for the implementation of control measures directed towards contagious agalactia. © 2007 Elsevier Ltd All rights reserved.

Classification Agris : L50 - Animal physiology and biochemistry

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

  • Woubit Salah, CIRAD-EMVT-UPR Contrôle des maladies (FRA)
  • Manso-Silvan Lucia, CIRAD-EMVT-UPR Contrôle des maladies (FRA) ORCID: 0000-0002-5539-3804
  • Lorenzon Sophie, CIRAD-EMVT-UPR Contrôle des maladies (FRA)
  • Gaurivaud Patrice, AFSSA (FRA)
  • Poumarat François, AFSSA (FRA)
  • Pellet Marie-Pierre, AFSSA (FRA)
  • Singh V.P., Indian Veterinary Research Institute (IND)
  • Thiaucourt François, CIRAD-EMVT-UPR Contrôle des maladies (FRA)

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