Successful nodulation of Casuarina by Frankia in axenic conditions

Echbab Hakima, Arahou Moustapha, Ducousso Marc, Nourissier-Mountou Sophie, Duponnois Robin, Lahlou Houria, Prin Yves. 2007. Successful nodulation of Casuarina by Frankia in axenic conditions. Journal of Applied Microbiology, 103 : pp. 1728-1737.

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Abstract : Aims: In order to depict the fine interactions that lead to nodulation, absolute microbiological control of the symbiotic partners is required, i.e. the ability to obtain in vitro axenic nodulation, a condition that has never been fulfilled with the Casuarina-Frankia symbiosis. The effects of culture conditions on plant growth and nodule formation by Casuarina cunninghamiana were investigated. Methods and Results: Axenic (capped tubes with different substrates), and nonaxenic cultures (Gibson tubes, pot cultures) were tested. In axenic conditions, C. cunninghamiana, inoculated with Frankia, had poor growth and did not form nodules at 6 weeks. Plants cultivated in Gibson tubes reached the four axillary shoots stage within 6 weeks and formed nodules 4 weeks after inoculation. Sand-pot cultures allowed us to relate the plant development stage at inoculation with nodulation. Conclusions: The sterile replacement of the cap by a plastic bag increased plant growth and enabled nodule formation 6 weeks after inoculation. The new system of plant culture allows the axenic nodule formation 6 weeks after inoculation. Nodulation behaviour is related to plant development and confinement. Significance and Impact of the Study: This axenic plant nodulation system is of major interest in analysing the roles of Frankia genes in nodulation pathways. (Résumé d'auteur)

Mots-clés Agrovoc : Casuarina cunninghamiana, Frankia, Culture in vitro, Fixation biologique de l'azote, nodosité racinaire, Formation de nodosités, Milieu de culture, Symbiose

Classification Agris : F62 - Plant physiology - Growth and development
P34 - Soil biology

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Echbab Hakima, Université Mohammed 5 (MAR)
  • Arahou Moustapha, Université Mohammed 5 (MAR)
  • Ducousso Marc, CIRAD-AMIS-UMR LSTM (FRA)
  • Nourissier-Mountou Sophie, CIRAD-FORET-UPR Génétique forestière (FRA)
  • Duponnois Robin, IRD (SEN)
  • Lahlou Houria, Université Mohammed 5 (MAR)
  • Prin Yves, CIRAD-AMIS-UMR LSTM (FRA) ORCID: 0000-0002-3706-0045

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