Evaluation of the mitochondrial COI gene as a useful genetic marker for Myndus crudus and other Myndus spp.

Brown J.K., Dollet Michel. 2007. Evaluation of the mitochondrial COI gene as a useful genetic marker for Myndus crudus and other Myndus spp.. Phytopathology, 97 (7), suppl., Résumé, S174

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Résumé : In the Americas and Caribbean, the lethal yellowing (LY) phytoplasma causes a disease of palms transmitted by the leafhopper Myndus crudus (family, Cixiidae), which may not be the sole vector. In Vanuatu and Ghana LY-like diseases also are rampant in coconut palm but M. taffini is thought to be a vector. Recently, high mortality in disease-resistant palms has occurred widely. The availability of a molecular marker would make possible the tracking of distribution and dispersal behaviors of Myndus species in relation to epidemic and non-epidemic areas. We employed PCR and mtCOI primers [F-C1-J-2195, R-L2-N-3014] to amplify from DNA extracts a 780 bp fragment. Phylogenetic analysis sorted putative Myndus spp. into three main clades: adiopodoumeensis (Ghana), crudus (Caribbean), and taffinii (Vanuvatu), the latter which comprised two sister groups. A fourth Myndus spp. from Oaxaca MEX grouped uniquely from other New World collections and herein has been identified as a new, putative vector species. (Texte intégral)

Mots-clés Agrovoc : Myndus, Myndus crudus, Myndus taffini, marqueur génétique, vecteur de maladie, phylogénie, identification, séquence nucléotidique, PCR, génétique mitochondriale

Mots-clés géographiques Agrovoc : Vanuatu, Ghana, République-Unie de Tanzanie

Mots-clés complémentaires : Jaunissement mortel, Myndus adiopodoumeensis

Classification Agris : H20 - Maladies des plantes
U30 - Méthodes de recherche

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Brown J.K., University of Arizona (USA)
• Dollet Michel, CIRAD-BIOS-UPR Etiologie dépérissements (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/542457/)

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