First complete chromosomal organization of a protozoan plant parasite (Phytomonas spp.)

Marin Clotilde, Alberge Blandine, Dollet Michel, Pagès Michel, Bastien Patrick. 2008. First complete chromosomal organization of a protozoan plant parasite (Phytomonas spp.). Genomics, 91 (1) : pp. 88-93.

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Abstract : Phytomonas spp. are members of the family Trypanosomatidae that parasitize plants and may cause lethal diseases in crops such as Coffee Phloem necrosis, Hartrot in coconut, and Marchitez sorpresiva in oil palm. In this study, the molecular karyotype of 6 isolates from latex plants has been entirely elucidated by pulsed-field gel electrophoresis and DNA hybridization. Twenty-one chromosomal linkage groups constituting heterologous chromosomes and sizing between 0.3 and 3 Mb could be physically defined by the use of 75 DNA markers (sequence-tagged sites and genes). From these data, the genome size can be estimated at 25.5 (±2) Mb. The physical linkage groups were consistently conserved in all strains examined. Moreover, the finding of several pairs of different-sized homologous chromosomes strongly suggest diploidy for this organism. The definition of the complete molecular karyotype of Phytomonas represents an essential primary step toward sequencing the genome of this parasite of economical importance. (Résumé d'auteur)

Mots-clés Agrovoc : Phytomonas, Génome, Chromosome, Caryotype, Hybridation moléculaire, Électrophorèse, Marqueur génétique, Euphorbia

Classification Agris : H20 - Plant diseases

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Marin Clotilde, Instituto de Biotecnologia (ESP)
  • Alberge Blandine, CNRS (FRA)
  • Dollet Michel, CIRAD-BIOS-UPR Etiologie dépérissements (FRA)
  • Pagès Michel, CNRS (FRA)
  • Bastien Patrick, CNRS (FRA)

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