Enzymatic production of conjugated linoleic acid monoacylglycerols from dehydrated isomerized castor bean oil

Barouh Nathalie, Piombo Georges, Goli Thierry, Baréa Bruno, Pina Michel, Lago Regina C.A., Villeneuve Pierre. 2008. Enzymatic production of conjugated linoleic acid monoacylglycerols from dehydrated isomerized castor bean oil. Journal of Food Lipids, 15 (1) : pp. 13-27.

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Abstract : The goal of this study was to produce conjugated linoleic acid (CLA) monoacylglycerols (MAGs). A chemicoenzymatic process was employed starting from castor bean oil, which was chemically dehydrated and isomerized to obtain new modified triacylglycerols (TAGs) with a very high proportion of CLA fatty acids (>95%). Such TAGs were then used in enzymatic glycerolysis reaction using Candida antarctica B lipase at 55C in tert-butyl alcohol as a solvent. The effect of enzyme load and substrate molar ratio on the production yield of MAGs was evaluated and optimized using response surface methodology with a two-factor and three-level factorial design. A satisfactory linear correlation existed between experimental results and predicted values (R2 = 0.97 and R2adj. = 0.94). Using the contour plot generated from the model for MAG production yield, optimum parameters could be obtained corresponding to an 11/1 glycerol/TAG molar ratio and an enzyme load of 2% (% w/w of both substrates). (Résumé d'auteur)

Mots-clés Agrovoc : Huile de ricin, Ricinus communis, Acide linoléique, Biosynthèse, Triacylglycérol lipase, Triglycéride, Candida, Acylglycérole, Activité enzymatique, Isomérisation, Séchage

Mots-clés complémentaires : Candida antarctica

Classification Agris : Q02 - Food processing and preservation
Q60 - Processing of non-food or non-feed agricultural products

Champ stratégique Cirad : Axe 3 (2005-2013) - Alimentation accessible et de qualité

Auteurs et affiliations

  • Barouh Nathalie, CIRAD-PERSYST-UMR IATE (FRA)
  • Piombo Georges, CIRAD-PERSYST-UMR IATE (FRA)
  • Goli Thierry, CIRAD-PERSYST-UMR Qualisud (FRA)
  • Lago Regina C.A., CIRAD-AMIS-UMR IATE (FRA)
  • Villeneuve Pierre, CIRAD-PERSYST-UMR IATE (FRA) ORCID: 0000-0003-1685-1494

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