Characterization of typo-, regio-, and stereo-selectivities of babaco latex lipase in aqueous and organic media

Cambon Emmanuelle, Pina Michel, Arondel Vincent, Rodriguez J.A., Carrière Frédéric, Turon Fabrice, Ruales Jenny, Villeneuve Pierre. 2008. Characterization of typo-, regio-, and stereo-selectivities of babaco latex lipase in aqueous and organic media. Biotechnology Letters, 30 (4) : pp. 769-774.

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Abstract : The unripe fruit of babaco (Vasconcellea 9 heilbornii; syn. Carica pentagona) contains a latex, similar to that in Carica papaya, which exhibits lipolytic activity. Herein, the regioselectivity, stereoselectivity and typoselectivity in both hydrolysis and acyltransfer reactions of babaco latex lipases were studied and compared to those of Carica papaya latex. In hydrolysis, both biocatalysts are 1,3-regioselective with ratios for 1,2-2,3-diacylglycerols/1,3- diacylglycerol of 6.5 and 21 for babaco and papaya, respectively. In contrast, papaya latex had a slight sn- 3 stereopreference. Babaco latex displayed a higher activity on triacylglycerols with short chain and unsaturated fatty acids. (Résumé d'auteur)

Mots-clés Agrovoc : Carica pentagona, Carica papaya, Latex, Estérase, Propriété physicochimique, Triacylglycérol lipase, Structure chimique, Biocatalyseur, Activité enzymatique, Acylation, Hydrolyse enzymatique, Lipase

Mots-clés géographiques Agrovoc : Équateur

Mots-clés complémentaires : Régiosélectivité, Sélectivité

Classification Agris : Q02 - Food processing and preservation

Champ stratégique Cirad : Hors axes (2005-2013)

Auteurs et affiliations

  • Cambon Emmanuelle, CIRAD-AMIS-UMR IATE (FRA)
  • Arondel Vincent, CNRS (FRA)
  • Rodriguez J.A., CNRS (FRA)
  • Carrière Frédéric, CNRS (FRA)
  • Turon Fabrice, CIRAD-AMIS-UMR IATE (FRA)
  • Ruales Jenny, EPN (ECU)
  • Villeneuve Pierre, CIRAD-PERSYST-UMR IATE (FRA) ORCID: 0000-0003-1685-1494

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