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Genetic maps of clementine mandarin and intergeneric hybrid clementine X poncirus using genomic and EST microsatellite markers

Luro François, Costantino Gilles, Billot Claire, Froelicher Yann, Morillon Raphaël, Ollitrault Patrick, Terol Javier F., Talon Manuel, Gmitter Frederick G. Jr., Chen Chunxian. 2007. Genetic maps of clementine mandarin and intergeneric hybrid clementine X poncirus using genomic and EST microsatellite markers. In : Abstracts of Plant and Animal Genomes XVth Conference, San Diego, CA (USA), January 13-17, 2007. s.l. : s.n., Résumé Plant and Animal Genomes Conference. 15, San Diego, États-Unis, 13 January 2007/17 January 2007.

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Abstract : A genetic map is a necessary first step to guide plant breeding programs and to explore the genome structure by localizing genes involved in important agronomic traits expression. For citrus, some genetic maps have been published but more often they were unsaturated or they employed dominant markers, poorly transferable to other labs. We have initiated a collaborative action between INRA (France), CIRAD (France), IVIA (Spain) and the University of Florida (USA) to generate a reliable and saturated genetic map of the Clementine genome with co-dominant markers localized in coding sequences. For this goal, we have investigated the presence of microsatellite sequences (SSRs) inside EST sequences from different libraries developed for sweet orange and Clementine. Nearly 10% of the ESTs include at least one SSR. By this way we expect to map directly genes whose function is identified by sequence comparison with GenBank or NCBI accessions. A segregating population of 88 individuals was created by INRA at San Giuliano by backcrossing an intergeneric hybrid (Clementine x Poncirus trifoliata) with Clementine. Due to the heterozygosity of the two parents (62% and 84% for Clementine and the intergeneric hybrid respectively), the efficiency of polymorphic EST-SSR markers tends to 45%. We have studied the segregation of more than 200 EST-SSR markers, and complemented with 75 genomic SSR markers previously developed we have constructed a genetic map. (Texte intégral)

Mots-clés Agrovoc : Clémentine, Citrus, Poncirus, Hybride, Carte génétique, Microsatellite, Marqueur génétique, Génome, Citrus reticulata, Citrus aurantium, Coopération internationale, Projet de recherche

Mots-clés complémentaires : Hybride intergénérique

Classification Agris : F30 - Plant genetics and breeding

Auteurs et affiliations

  • Luro François, INRA (FRA)
  • Costantino Gilles, INRA (FRA)
  • Billot Claire, CIRAD-BIOS-UMR DAP (FRA)
  • Froelicher Yann, CIRAD-BIOS-UPR Multiplication végétative (FRA)
  • Morillon Raphaël, CIRAD-BIOS-UPR Multiplication végétative (ESP)
  • Ollitrault Patrick, CIRAD-BIOS-UPR Multiplication végétative (ESP) ORCID: 0000-0002-9456-5517
  • Terol Javier F., IVIA (ESP)
  • Talon Manuel, IVIA (ESP)
  • Gmitter Frederick G. Jr., University of Florida (USA)
  • Chen Chunxian, University of Florida (USA)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/543999/)

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