Agritrop
Home

The low-virulent African swine fever virus (ASFV/NH/P68) induces enhanced expression and production of relevant regulatory cytokines (IFNa, TNFa and IL12p40) on porcine macrophages in comparison to the highly virulent ASFV/L60

Gil S., Sepulveda N., Albina Emmanuel, Leitão A., Martins C.. 2008. The low-virulent African swine fever virus (ASFV/NH/P68) induces enhanced expression and production of relevant regulatory cytokines (IFNa, TNFa and IL12p40) on porcine macrophages in comparison to the highly virulent ASFV/L60. Archives of Virology, 153 (10) : pp. 1845-1854.

Journal article ; Article de revue à facteur d'impact
[img] Published version - Anglais
Access restricted to CIRAD agents
Use under authorization by the author or CIRAD.
document_547281.pdf

Télécharger (301kB)

Abstract : The impact of infection by the low-virulent ASFV/NH/P68 (NHV) and the highly virulent ASFV/L60 (L60) isolates on porcine macrophages was assessed through the quantification of IFNa, TNFa, IL12p40, TGFb and ASFV genes by real-time PCR at 2, 4 and 6 h postinfection. Increased IFNa, TNFa and IL12p40 expression was found in infection with NHV, in which expression of TGFb was lower than in infection with L60. Principal component analysis showed a positive interaction of cytokines involved in cellular immune mechanisms, namely IFNa and IL12p40 in the NHV infection. Quantification by ELISA confirmed higher production of IFNa, TNFa and IL12p40 in the NHV-infected macrophages. Overall, our studies reinforce and clarify the effect of the NHV infection by targeting cellular and cellular-based immune responses relevant for pig survival against ASFV infection. (Résumé d'auteur)

Mots-clés Agrovoc : Virus peste porcine africaine, Peste porcine africaine, Cytokinine, Infection, Pouvoir pathogène, Macrophage

Classification Agris : L73 - Animal diseases

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

  • Gil S., Technical University of Lisbon (PRT)
  • Sepulveda N., Technical University of Lisbon (PRT)
  • Albina Emmanuel, CIRAD-BIOS-UMR CMAEE (FRA)
  • Leitão A., Technical University of Lisbon (PRT)
  • Martins C., Technical University of Lisbon (PRT)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/547281/)

View Item (staff only) View Item (staff only)

[ Page générée et mise en cache le 2021-03-07 ]