Characterization of T-ceell memory responses associated with control of CBPP and identification of immuno-active proteins of Mmm

Totté Philippe, Mather Arshad, Reslan Lina, Rodrigues Valérie, Viley Eddy, Frey Joachim, Du Plessis Dion H., Dedieu Laurence. 2008. Characterization of T-ceell memory responses associated with control of CBPP and identification of immuno-active proteins of Mmm. In : 17th Congress of the International Organization For Mycoplasmology, July 6-11, 2008, Tianji, China. s.l. : s.n., Résumé, 1 p. Congress of the International Organization For Mycoplasmology. 17, Tianjin, Chine, 6 July 2008/11 July 2008.

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Abstract : A better understanding of the basis of protective immunity and immunopathologic reactions associated with contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp. mycoides small colony (MmmSC), is needed to develop better vaccines. Here we report on the characterization of T-cell memory responses associated with protective immunity in cattle and their use thereof to identify immuno-active proteins of MmmSC. Both effector memory (em) and central memory (cm)-like MmmSC specific CD4+ T cells were present ex vivo and capable of proliferation and IFN-[gamma] production upon re-stimulation in vitro with inactivated MmmSC. It has been shown in mice and humans that priming of T(cm) is required for long-lived protective immunity, which is also an essential requirement of any improved vaccine against CBPP. Pre-sensitized T-cells were used to screen recombinant proteins of MmmSC produced as his-tagged proteins_and purified by chromatography. Three of them were found to trigger the proliferation of both CD4+ T(em) and T(cm) and induce the production of IFN-[gamma]: lipoprotein A (LppA), a glucose-specific component of the PTS system (ptsG) and to a lesser extent a substrate-binding component of the ABC transporter (ABC). In contrast, although 1ppQ had no effect on its own, it strongly inhibited the proliferation of CD4 in response to MmmSC by a mechanism apparently independent of direct cytotoxicity. In conclusion, the T-cell reagents and methodology described in this study will help identify immuno-protective antigens of MmmSC that have potential for the development of a subunit vaccine against CBPP. In addition, these cellular tools can also be used to screen for immuno-inhibitory proteins whose corresponding genes could be targets for deletion/inactivation in the aim of developing improved attenuated vaccines. (Texte intégral)

Mots-clés Agrovoc : Mycoplasma mycoides, Bovin

Classification Agris : L73 - Animal diseases

Auteurs et affiliations

  • Totté Philippe, CIRAD-BIOS-UPR Contrôle des maladies (FRA)
  • Mather Arshad, OVI (ZAF)
  • Reslan Lina
  • Rodrigues Valérie, CIRAD-BIOS-UMR CMAEE (FRA)
  • Viley Eddy, Institut de bactériologie vétérinaire (CHE)
  • Frey Joachim, Institut de bactériologie vétérinaire (CHE)
  • Du Plessis Dion H., OVI (ZAF)
  • Dedieu Laurence, CIRAD-BIOS-UPR Contrôle des maladies (FRA) ORCID: 0000-0002-6492-3002

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