Species identification, molecular sexing and genotyping using non-invasive approaches in two wild bovids species: Bos gaurus and Bos javanicus

Rivière-Dobigny Taiana, Pham Doan Lan, Le Quang Nam, Maillard Jean-Charles, Michaux Johan. 2009. Species identification, molecular sexing and genotyping using non-invasive approaches in two wild bovids species: Bos gaurus and Bos javanicus. Zoo Biology, 28 (2) : pp. 127-136.

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Quartile : Q3, Sujet : VETERINARY SCIENCES / Quartile : Q4, Sujet : ZOOLOGY

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Abstract : Since the second Indochina war, habitat destruction and overhunting has resulted in fragmentation of the remaining populations of Bos javanicus and B. gaurus. Nowadays, both species are in serious danger, especially the gaur. In Vietnam, where these species have become almost impossible to capture in the wild, noninvasive investigations are the only feasible approach to obtain data on populations. However, non-invasive derived DNA, especially in tropical areas, is usually characterized by low concentrations, poor quality and/or contamination from alien DNA. To assist in tropical conservation management, baseline information is provided here on assessing the reliability of species identification, molecular sexing and microsatellite genotyping using fecal DNA from B. gaurus and B. javanicus. For species identification using bovine fecal samples, cytochrome b fragment between positions 867 and 1140 was found to contain species diagnostic sites, which distinguishes the four species encountered in the region: B. gaurus, B. indicus, B. javanicus and B. taurus. For sex determination, primers were initially tested on DNA obtained from blood. Then, these primers were successfully used on DNA derived from fecal material. Finally, we also evaluate the feasibility of non-invasive microsatellite genotyping on fecal samples collected in Vietnamese nature reserves. The results presented here improve on current molecular methods based on fecal material obtained from tropical areas. (Résumé d'auteur)

Mots-clés Agrovoc : Gaur, Banteng, Animal sauvage

Mots-clés géographiques Agrovoc : France, Viet Nam

Classification Agris : U30 - Research methods
F30 - Plant genetics and breeding
L20 - Animal ecology
L60 - Animal taxonomy and geography

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Rivière-Dobigny Taiana
  • Pham Doan Lan, NIAH (VNM)
  • Le Quang Nam, NIAH (VNM)
  • Maillard Jean-Charles, CIRAD-ES-UPR Faune sauvage (VNM)
  • Michaux Johan, Montpellier SupAgro (FRA)

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