Temperature-induced male differentiation in the Nile Tilapia : gonad gene expression using female monosex populations and divergent thermo-sensitive lines

D'Cotta Helena, Pepey Elodie, Wessels Stephan, Poonlaphdecha Srisupaph, Reinelt Birgit, Horstgen-Schwark Gabriele, Baroiller Jean-François. 2008. Temperature-induced male differentiation in the Nile Tilapia : gonad gene expression using female monosex populations and divergent thermo-sensitive lines. Sexual Development, 2, Résumé : p. 281.

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Abstract : Sex in the Nile tilapia, Oreochromis niloticus, is governed by the interactions between a complex genetic sex determination system and the influence of temperature. High temperatures applied during a critical period of sex differentiation can induce masculinisation in some progenies originating from both domestic and wild stocks. Differences in thermo-sensitivity were observed between the wild populations and both paternal and maternal effects have been demonstrated, suggesting that it is a heritable trait. This has been confirmed by the development of divergent lines for thermo-sensitivity. Two thermo-sensitive divergent lines were selected with regard to their sex ratios showing high (=90% males) and low responses (only 54%) when treated at 36°C temperatures. At least one of the mechanisms by which temperature exerts its effects on sex differentiation is through a downregulation of aromatase Cyp19. Expression profiles were analysed in monosex XX populations treated at 27°C and 36°C temperatures showing that temperature up-regulates Sox9s, Amh, IGFs, Dax1 and down-regulates other genes such as aromatase Cyp19-1a. Variation in these gene expression levels was associated with the percentage of males obtained by temperature treatments. The divergent thermo-sensitive lines have been used to complement our study done on the monosex populations. Expression of Cyp19-1a was also analysed in these two divergent lines. (Texte intégral)

Mots-clés Agrovoc : Oreochromis niloticus, Détermination du sexe, Différenciation sexuelle, Température, Expression des gènes, Femelle

Classification Agris : M12 - Aquaculture production and management
L53 - Animal physiology - Reproduction
L10 - Animal genetics and breeding

Axe stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • D'Cotta Helena, CIRAD-PERSYST-UPR Aquaculture (FRA)
  • Pepey Elodie, CIRAD-PERSYST-UPR Aquaculture (FRA)
  • Wessels Stephan, CIRAD-PERSYST-UPR Aquaculture (FRA)
  • Poonlaphdecha Srisupaph, CIRAD-PERSYST-UPR Aquaculture (FRA)
  • Reinelt Birgit, Institute of Animal Husbandry and Genetics (DEU)
  • Horstgen-Schwark Gabriele, Institute of Animal Husbandry and Genetics (DEU)
  • Baroiller Jean-François, CIRAD-PERSYST-UPR Aquaculture (FRA) ORCID: 0000-0002-0992-1725

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