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New molecular method for the early detection of wood fungi using DNA in utility wood poles

Zaremski Alba, Gastonguay Louis, Lessard-Deziel Denise, Robitaille Michel, Chaintreuil Clémence, Prin Yves. 2009. New molecular method for the early detection of wood fungi using DNA in utility wood poles. In : 7th International Conference on Wood Science and Engineering in the Third Millennium, June 4-6, 2009, Brasov, Romania. s.l. : s.n., pp. 542-545. International Conference : Wood Science and Engineering in the Third Millennium. 7, Brasov, Roumanie, 4 June 2009/6 June 2009.

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Abstract : Presently, there is no direct method for the early detection of fungi in wood samples. With the increase importance of wood decay by micro-organisms in the forest industry, there is a need to develop a test that is fast, easy to use, specific, portable, automate and with a low detection limit. A technique developed in the CIRAD laboratory has all the desire requirements; 1) Using PCR (polymerise chain reaction), the technique is able to specifically amplify a DNA sequence of fungus; 2) it combines the sensitivity and accuracy of the PCR with the use of an international bank of nucleic sequences. This method, of fungi molecular detection, is routinely used in the CIRAD laboratory in order to identify fungi responsible for the production of rot in lumber. The method was used and adapts in order to measure the presence of fungi in wood poles, of different ages, from the distribution network of Hydro-Québec in Canada. (Résumé d'auteur)

Mots-clés Agrovoc : Bois

Classification Agris : K50 - Processing of forest products
U40 - Surveying methods
J12 - Handling, transport, storage and protection of forest products

Auteurs et affiliations

  • Zaremski Alba, CIRAD-BIOS-UPR Génétique forestière (FRA)
  • Gastonguay Louis
  • Lessard-Deziel Denise
  • Robitaille Michel
  • Chaintreuil Clémence
  • Prin Yves, CIRAD-BIOS-UMR LSTM (FRA) ORCID: 0000-0002-3706-0045

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/550907/)

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