Evidence of unreduced gamete production from interspecific crosses between Gossypium hirsutum and G. herbaceum

Pannetier Catherine, Hofs Jean-Luc, Montes Emilie, Eber Frédéric, Coriton Olivier, Huteau Virginie, Hau Bernard, Chèvre Anne-Marie. 2009. Evidence of unreduced gamete production from interspecific crosses between Gossypium hirsutum and G. herbaceum. In : International Conference on Polyploidy, Hybridization and Biodiversity, May 17 - 20, 2009, Saint-Malo, France, program and abstracts. Université de Rennes 1. Rennes : Université de Rennes 1, Résumé, p. 178. International Conference on Polyploidy, Hybridization and Biodiversity, Saint-Malo, France, 17 May 2009/20 May 2009.

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Abstract : In order to analyze the gene flow between the allotetraploid cultivated cotton (Gossypium hirsutum, AADD, 2n=52) and the wild diploid (G. herbaceum, AA, 2n=26), the possibility of natural hybridization between these two cotton species has been investigated. In fact, in South Africa and particularly in the KwaZulu Natal Province, where commercialisation of transgenic Bt cotton began in 1998, a wild species (G. herbaceum) is neighbouring from the cultivated cotton fields. From reciprocal crosses performed without emasculation between G herbaceum used as female or male and G hirsutum, 148 and 335 plants respectively, have been analyzed. Neither examination of the morphological characteristics nor the flow cytometry analysis of the 335 plants resulting from the cross G hirsutum x G herbaceum, have shown any to be hybrid plants. For the cross G herbaceum x G hirsutum, three plants have shown a hybrid phenotype. Analysis of DNA content by flow cytometry and morphological traits, have clearly shown that two of them were triploid (AAD). The third one exhibited a value in flow cytometry slightly higher than G hirsutum. In addition some morphological characteristics (plant morphology, presence and size of petal spots, leaf shape...) have led us to conclude that this plant is AAAD and was the result of a fecundation with unreduced gamete AA from the female G herbaceum parent. Fluorescent In Situ Hybridization (FISH), and meiotic behaviour have confirmed this hypothesis. This is, to our knowledge, the first description of the occurrence of a non-reductional meiosis in the species G. herbaceum. This plant material could provide a useful tool for the study of the expression of genes duplicated in the A and D cotton genome. The possibility of obtaining an interspecific hybrid between cultivated and diploid wild cotton through fertilization with an unreduced gamete raises the question of its evolution in natural populations. (Texte intégral)

Mots-clés Agrovoc : Gossypium hirsutum, Gossypium herbaceum

Classification Agris : F30 - Plant genetics and breeding
F63 - Plant physiology - Reproduction

Auteurs et affiliations

  • Pannetier Catherine, CIRAD-PERSYST-UPR SCA (FRA)
  • Montes Emilie, INRA (FRA)
  • Eber Frédéric, INRA (FRA)
  • Coriton Olivier, INRA (FRA)
  • Huteau Virginie, INRA (FRA)
  • Hau Bernard
  • Chèvre Anne-Marie, INRA (FRA)

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