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Molecular diagnostics in the Mycosphaerella leaf spot disease complex of banana and for Radopholus similis

Arzanlou Mahdi, Kema Gert H.J., Waalwijk Cees, De Vries Ineke, Araya Vargas M., Helder J., Crous Pedro W.. 2009. Molecular diagnostics in the Mycosphaerella leaf spot disease complex of banana and for Radopholus similis. In : Proceedings of the international symposium on recent advances in banana crop protection for sustainable production and improved livelihoods, White River, South Africa, September 10-14, 2007. Jones David R. (ed.), Van den Bergh Inge (ed.). ISHS. Louvain : ISHS [Belgique], pp. 237-243. (Acta Horticulturae, 828) ISBN 978-90-6605-488-2 International Symposium on recent advances in banana crop protection for sustainable production and improved livelihoods. 1, White River, Afrique du Sud, 10 September 2007/14 September 2007.

Paper with proceedings
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Abstract : Mycosphaerella leaf spots and nematodes threaten banana cultivation worldwide. The Mycosphaerella disease complex involves three related ascomycetous fungi: #Mycosphaerella fijiensis#, #M. musicola# and #M. eumusae#. The exact distribution of these three species and their disease epidemiology remain unclear, since their symptoms and life cycles are rather similar. Diagnosing these diseases and the respective causal agents is based on the presence of host symptoms and fungal fruiting structures, but is time consuming and not conducive to preventive management. In the present study, we developed rapid and robust species-specific diagnostic tools to detect and quantify #M. fijiensis#, #M. musicola# and #M. eumusae#. Conventional species-specific PCR primers were developed based on the actin gene that detected as little as 100, 1 and 10 pg/µl DNA from, respectively, #M. fijiensis#, #M. musicola# and #M. eumusae#. Furthermore, TaqMan real-time quantitative PCR assays that were developed based on the ß-tubulin gene detected quantities as low as 1 pg/µl DNA of each species from pure cultures and 1.6 pg/µl DNA/mg of #M. fijiensis# from dry leaf tissue. The efficacy of the tests was validated using naturally infected banana leaves. Similar technology has been used to develop a quantitative PCR assay for the banana burrowing nematode, #Radopholus similis#, which is currently being validated. (Résumé d'auteur)

Mots-clés Agrovoc : Musa, Radopholus similis, Mycosphaerella fijiensis, Mycosphaerella musicola, Maladie des raies noires

Mots-clés complémentaires : Mycosphaerella eumusae

Classification Agris : H20 - Plant diseases
F30 - Plant genetics and breeding

Auteurs et affiliations

  • Arzanlou Mahdi, CBS (NLD)
  • Kema Gert H.J., PRI (NLD)
  • Waalwijk Cees, PRI (NLD)
  • De Vries Ineke
  • Araya Vargas M., CORBANA (CRI)
  • Helder J., Wageningen University (NLD)
  • Crous Pedro W., CBS (NLD)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/552620/)

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