Micropropagation by tissue culture triggers differential expression of infectious endogenous Banana streak virus sequences (eBSV) present in the B genome of natural and synthetic interspecific banana plantains

Côte François-Xavier, Galzi Serge, Folliot Michel, Lamagnère Yannick, Teycheney Pierre-Yves, Iskra Caruana Marie-Line. 2010. Micropropagation by tissue culture triggers differential expression of infectious endogenous Banana streak virus sequences (eBSV) present in the B genome of natural and synthetic interspecific banana plantains. Molecular Plant Pathology, 11 (1) : 137-144.

Résumé : The genome of Musa balbisiana spp. contains several infectious endogenous sequences of Banana streak virus (eBSV). We have shown previously that in vitro micropropagation triggers the activation of infectious eBSOLV (endogenous sequences of Banana streak Obino l'Ewai virus) in the synthetic tetraploid interspecific hybrid FHIA21 (AAAB). In this work, we show that another synthetic tetraploid (AAAB) hybrid and two natural triploid (AAB) plantains are equally prone to the activation of infectious eBSOLV during tissue culture. These results are a strong indication that such activation is a general phenomenon in interspecific Musa cultivars, whether synthetic or natural.We also report the first in-depth study of the correlation between the duration of tissue culture and the level of activation of infectious eBSOLV, and show that specific and common activation patterns exist in these banana plants.We hypothesize that these patterns result from the concomitant activation of infectious eBSOLV and a decrease in the virus titre in neoformed plantlets, resulting from cell multiplication outcompeting virus replication. We provide experimental data supporting this hypothesis. No activation of infectious eBSGFV (endogenous sequences of Banana streak Goldfinger virus) by tissue culture was observed in the two natural AAB plantain cultivars studied here, whereas such activation occurred in the AAAB synthetic hybrid studied. We demonstrate that this differential activation does not result from differences in the structure of eBSGFV, as all banana genomes harbour eaBSGFV-7.

Mots-clés Agrovoc : Musa, Musa balbisiana, virus des végétaux, multiplication végétative, culture de tissu, génome

Mots-clés complémentaires : Banana streak virus, Pararétrovirus, Endovirus

Classification Agris : H20 - Maladies des plantes
F02 - Multiplication végétative des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Côte François-Xavier, CIRAD-PERSYST-UPR Systèmes bananes et ananas (FRA)
• Galzi Serge, CIRAD-BIOS-UMR BGPI (FRA)
• Folliot Michel
• Lamagnère Yannick, Vitropic (FRA)
• Teycheney Pierre-Yves, CIRAD-BIOS-UPR Multiplication végétative (GLP) ORCID: 0000-0002-9754-0745
• Iskra Caruana Marie-Line, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0003-4486-2449

Source : Cirad - Agritrop (https://agritrop.cirad.fr/552721/)

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