A grapevine Shaker inward K+ channel activated by the calcineurin B-like calcium sensor 1-protein kinase CIPK23 network is expressed in grape berries under drought stress conditions

Cuellar Térésa, Pascaud François, Verdeil Jean-Luc, Torregrosa Laurent, Adam-Blondon Anne-Françoise, Thibaud Jean-Baptiste, Sentenac Hervé, Gaillard Isabelle. 2010. A grapevine Shaker inward K+ channel activated by the calcineurin B-like calcium sensor 1-protein kinase CIPK23 network is expressed in grape berries under drought stress conditions. Plant Journal, 61 (1) : 58-69.

Quartile : Outlier, Sujet : PLANT SCIENCES

Résumé : Grapevine (Vitis vinifera), the genome sequence of which has recently been reported, is considered as a model species to study fleshy fruit development and acid fruit physiology. Grape berry acidity is quantitatively and qualitatively affected upon increased K+ accumulation, resulting in deleterious effects on fruit (and wine) quality. Aiming at identifying molecular determinants of K+ transport in grapevine, we have identified a K+ channel, named VvK1.1, from the Shaker family. In silico analyses indicated that VvK1.1 is the grapevine counterpart of the Arabidopsis AKT1 channel, known to dominate the plasma membrane inward conductance to K+ in root periphery cells, and to play a major role in K+ uptake from the soil solution. VvK1.1 shares common functional properties with AKT1, such as inward rectification (resulting from voltage sensitivity) or regulation by calcineurin B-like (CBL)-interacting protein kinase (CIPK) and Ca2+-sensing CBL partners (shown upon heterologous expression in Xenopus oocytes). It also displays distinctive features such as activation at much more negative membrane voltages or expression strongly sensitive to drought stress and ABA (upregulation in aerial parts, downregulation in roots). In roots, VvK1.1 is mainly expressed in cortical cells, like AKT1. In aerial parts, VvK1.1 transcripts were detected in most organs, with expression levels being the highest in the berries. VvK1.1 expression in the berry is localized in the phloem vasculature and pip teguments, and displays strong upregulation upon drought stress, by about 10-fold. VvK1.1 could thus play a major role in K+ loading into berry tissues, especially upon drought stress.

Mots-clés Agrovoc : Vitis vinifera, Arabidopsis, fruits à pépins, potassium, transport des substances nutritives, génome, expression des gènes, physiologie végétale, stress osmotique, stress dû à la sécheresse, hybridation

Classification Agris : F62 - Physiologie végétale - Croissance et développement
F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Cuellar Térésa, CIRAD-BIOS-UMR DAP (FRA)
• Pascaud François, CNRS (FRA)
• Verdeil Jean-Luc, CIRAD-BIOS-UMR DAP (FRA)
• Torregrosa Laurent, INRA (FRA)
• Adam-Blondon Anne-Françoise, INRA (FRA)
• Thibaud Jean-Baptiste, CNRS (FRA)
• Sentenac Hervé, CNRS (FRA)
• Gaillard Isabelle, CNRS (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/552773/)

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