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Morpho-histological study of banana (Musa spp.cv. Grande naine [AAA]) cell suspensions during cryopreservation and regeneration

Georget Frederic, Engelmann Florent, Domergue Régis, Côte François-Xavier. 2009. Morpho-histological study of banana (Musa spp.cv. Grande naine [AAA]) cell suspensions during cryopreservation and regeneration. CryoLetters, 30 (6) : pp. 398-407.

Journal article ; Article de revue à facteur d'impact
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Quartile : Q3, Sujet : BIOLOGY / Quartile : Q4, Sujet : PHYSIOLOGY

Abstract : In this work, a morpho-histological study of banana (Musa spp. cv. Grande Naine [AAA]) embryogenic cell suspensions during cryopreservation and regeneration was performed. It was demonstrated that the regeneration process of somatic embryos originating from cryopreserved cell suspensions was different from that of control cell suspensions. Somatic embryos originating from cryopreserved cell suspensions had a unicellular origin. The regeneration process was modified not only by freezing in liquid nitrogen but also by the plasmolyzing effect of the 0.5 M sucrose solution employed during pretreatment. This result explained the high number of embryonic structures formed on M3 medium, compared with the control. Proembryos blocked at the globular stage could pursue their development when they were plated on new culture medium at a lower density after 30 days of culture on M3 medium. The unicellular origin of somatic embryos produced from cryopreserved cell suspensions offers the prospect of using cryopreservation to select non-chimeral transformed plants. (Résumé d'auteur)

Mots-clés Agrovoc : Musa, Embryon végétal, Cryoconservation, histology [EN], Sélection in vitro, Embryon somatique, Régénération in vitro

Mots-clés géographiques Agrovoc : Languedoc-Roussillon

Classification Agris : F62 - Plant physiology - Growth and development
F02 - Plant propagation
F30 - Plant genetics and breeding

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Georget Frederic, CIRAD-BIOS-UMR RPB (NIC)
  • Engelmann Florent, IRD (FRA)
  • Domergue Régis, CIRAD-PERSYST-UPR Systèmes bananes et ananas (FRA)
  • Côte François-Xavier, CIRAD-PERSYST-UPR Systèmes bananes et ananas (FRA)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/554331/)

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