Progress on Eucalyptus BAC libraries characterization

Paiva Jorge, Vautrin Sonia, Prat Elisa, Santos Mauro, Araujo Susana, Fonseca Paula, Gion Jean-Marc, Fevereiro Pedro, Freitas Ana, Marques Cristina, Berges Hélène, Grima Pettenati Jacqueline. 2010. Progress on Eucalyptus BAC libraries characterization. In : Abstracts of Plant and Animal Genomes XVIIIth Conference, San Diego, CA (USA), January 09-13, 2010. s.l. : s.n., Résumé Plant and Animal Genomes Conference. 18, San Diego, États-Unis, 9 January 2010/13 January 2010.

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Abstract : Eucalyptus species are amongst the most planted hardwoods in the world. However, because of their long generation times as compared to crop species, they are still at the early stages of domestication. Genomic technologies could significantly speed up the process of genetic improvement of Eucalyptus. Genomic libraries are important genomic tools suitable for physical mapping, map-based cloning and genome sequencing. Here we present the results of the progress on analysis of BAC libraries for E. grandis and E. globulus, the two main commercial Eucalyptus species. For E. globulusE. grandis BAC clone, positive for chloroplast genome, was full sequenced with 454 pyrosequencing technology. The E. grandis chloroplast consists of 160,137bp and is 99% identical to that of E. globulus chloroplast genome sequence. These genomes are very well conserved in gene content and structure. A dedicated Eucalyptus genomic database was implemented to stock and manage all data produced. Results on BAC libraries characterization obtained in the frame of GenEglobwq project (FCT funding, Portugal) and within the Portugal-France collaborative research efforts (EUCABAC) will be presented and discussed. (Texte intégral)

Classification Agris : F30 - Plant genetics and breeding
C30 - Documentation and information
K01 - Forestry - General aspects

Auteurs et affiliations

  • Paiva Jorge, IICT (PRT)
  • Vautrin Sonia, INRA (FRA)
  • Prat Elisa, INRA (FRA)
  • Santos Mauro, INESC (PRT)
  • Araujo Susana, IICT (PRT)
  • Fonseca Paula, INESC (PRT)
  • Gion Jean-Marc, CIRAD-BIOS-UPR Génétique forestière (FRA)
  • Fevereiro Pedro, IBET (PRT)
  • Freitas Ana, INESC (PRT)
  • Marques Cristina, Forestry and Paper Research Institute (PRT)
  • Berges Hélène, INRA (FRA)
  • Grima Pettenati Jacqueline, CNRS (FRA)

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