Characterisation of a cyclin-dependent kinase (CDKA) gene expressed during somatic embryogenesis of coconut palm

Montero-Cortés Mayra, Rodriguez-Paredes Francisco, Burgeff Caroline, Pérez-Nuñez Teresa, Cordova Ivan, Oropeza Carlos, Verdeil Jean-Luc, Saenz Luis. 2010. Characterisation of a cyclin-dependent kinase (CDKA) gene expressed during somatic embryogenesis of coconut palm. Plant Cell, Tissue and Organ Culture, 102 (2) : pp. 251-258.

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Abstract : The CDKA gene is linked to the cellular control. This gene was isolated from coconut palm (Cocos nucifera L) and a detailed expression analysis was done during somatic embryogenesis. Analysis of the deduced amino acid sequence showed the most important residues to be conserved. The highest homology was with Picea abies (96% similarity). Expression of the putative CnCDKA gene steadily increased during embryogenic callus formation phase when embryogenic competence is attained. In situ hybridization specified the localization of the transcripts as being mainly in a few cell layers within the meristematic centres in embryogenic calli at 90 day cultures. Analysis of CnCDKA expression at different somatic embryo formation stages showed that the expression was decreased progressively with the lowest expression in germinated somatic embryos. (Résumé d'auteur)

Mots-clés Agrovoc : Cocos nucifera

Classification Agris : F30 - Plant genetics and breeding
F63 - Plant physiology - Reproduction
F62 - Plant physiology - Growth and development
F02 - Plant propagation

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Montero-Cortés Mayra, CICY (MEX)
  • Rodriguez-Paredes Francisco, CICY (MEX)
  • Burgeff Caroline, CICY (MEX)
  • Pérez-Nuñez Teresa, CICY (MEX)
  • Cordova Ivan, CICY (MEX)
  • Oropeza Carlos, CICY (MEX)
  • Verdeil Jean-Luc, CIRAD-BIOS-UMR DAP (FRA)
  • Saenz Luis, CICY (MEX)

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