Imaging plant growth in 4D: Robust tissue reconstruction and lineaging at cell resolution

Fernandez Romain, Das Pradeep, Mirabet Vincent, Moscardi Eric, Traas Jan, Verdeil Jean-Luc, Malandain Grégoire, Godin Christophe. 2010. Imaging plant growth in 4D: Robust tissue reconstruction and lineaging at cell resolution. Nature Methods, 7 (7) : pp. 547-553.

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Abstract : Quantitative information on growing organs is required to better understand morphogenesis in both plants and animals. However, detailed analyses of growth patterns at cellular resolution have remained elusive. We developed an approach, multiangle image acquisition, three-dimensional reconstruction and cell segmentation-automated lineage tracking (MARS-ALT), in which we imaged whole organs from multiple angles, computationally merged and segmented these images to provide accurate cell identification in three dimensions and automatically tracked cell lineages through multiple rounds of cell division during development. Using these methods, we quantitatively analyzed Arabidopsis thaliana flower development at cell resolution, which revealed differential growth patterns of key regions during early stages of floral morphogenesis. Lastly, using rice roots, we demonstrated that this approach is both generic and scalable. (Résumé d'auteur)

Classification Agris : U10 - Mathematical and statistical methods
F50 - Plant structure
F62 - Plant physiology - Growth and development

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Fernandez Romain, CIRAD-BIOS-UMR DAP (FRA)
  • Das Pradeep, ENS Lyon (FRA)
  • Mirabet Vincent, ENS Lyon (FRA)
  • Moscardi Eric, INRIA (FRA)
  • Traas Jan, ENS Lyon (FRA)
  • Verdeil Jean-Luc, CIRAD-BIOS-UMR DAP (FRA)
  • Malandain Grégoire, INRIA (FRA)
  • Godin Christophe, INRIA (FRA)

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