Ralstonia solanacearum induces soluble amine-oxidase activity in Solanum torvumstem calli

Aribaud Marcel, Jego Sylvaine, Wicker Emmanuel, Fock Isabelle. 2010. Ralstonia solanacearum induces soluble amine-oxidase activity in Solanum torvumstem calli. Plant Physiology and Biochemistry, 48 (9) : pp. 787-796.

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Quartile : Q1, Sujet : PLANT SCIENCES

Abstract : #Solanum torvum# is reported to carry resistance to bacterial wilt caused by #Ralstonia solanacearum#. So, this wild species is used as rootskock for eggplants or tomatoes in naturally infected soil. This study aimed to investigate the involvement of the polyamine metabolism pathway in the resistance mechanisms of this species. Calli induced from Solanum torvum stem explants were inoculated with the bacteria under partial vacuum. All calli showed a hypersensitive response after infiltration. Furthermore, amine oxidase activity with aldehyde and H2O2 production was detected in soluble protein extracts of calli infiltrated by the bacteria. Due to its preferential affinity for aliphatic amines, this enzyme was supposed to have amine oxidase-like (AO-like) activity. Moreover, the length of aliphatic chain cycle altered the oxidative deamination kinetics of potential substrates. The AO-like catalytic activity was significantly inhibited by chelator agents such as ethylene-diamine-tretraacetic (EDTA), and also by semi-carbazide as aminoguanidine. These results suggested that (i) the prosthetic group of the AO-like enzyme could be a tyrosine-derived 6-hydroxytopaquinone structure, copper containing; (ii) this enzyme could be a semi-carbazide sensitive amine oxidase (SSAO). (Résumé d'auteur)

Mots-clés Agrovoc : Ralstonia solanacearum, Solanum

Mots-clés géographiques Agrovoc : Réunion

Mots-clés complémentaires : Flétrissement bactérien

Classification Agris : H20 - Plant diseases

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Aribaud Marcel, Université de la Réunion (REU)
  • Jego Sylvaine, CIRAD-BIOS-UMR PVBMT (REU)
  • Wicker Emmanuel, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0003-0927-7404
  • Fock Isabelle, Université de la Réunion (REU)

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