Folate polyglutamylation is required for rice seed development

Anukul Nampeung, Ramos Riza Abilgos, Mehrshahi Payam, Castelazo Anahi Santoyo, Parker Hélène, Dievart Anne, Lanau Nadège, Mieulet Delphine, Tucker Grégory, Guiderdoni Emmanuel, Barret David A, Bennett Malcolm J.. 2010. Folate polyglutamylation is required for rice seed development. Rice, 3 : pp. 181-193. International Rice Genetics Symposium. 6, Manille, Philippines, 6 November 2009/19 November 2009.

Journal article ; Article de revue à facteur d'impact
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Quartile : Q1, Sujet : AGRONOMY

Abstract : In plants, polyglutamylated folate forms account for a significant proportion of the total folate pool. Polyglutamylated folate forms are produced by the enzyme folylpolyglutamate synthetase (FPGS). The FPGS enzyme is encoded by two genes in rice, Os03g02030 and Os10g35940. Os03g02030 represents the major expressed form in developing seed. To determine the function of this FPGS gene in rice, a T-DNA knockout line was characterised. Disrupting Os03g02030 gene expression resulted in delayed seed filling. LC-MS/MS-based metabolite profiling revealed that the abundance of mono- and polyglutamylated folate forms was significantly decreased in seeds of the knockout line. RT-qPCR detected an increase in the transcript abundance of folate biosynthesis genes in seed of the knockout plant, whereas the folate deglutamating enzyme ?-glutamyl hydrolase mRNA level was reduced. Our study has uncovered a novel role for folate polyglutamylation during rice seed development and a potential feedback mechanism to maintain folate abundance.(Résumé d'auteur)

Mots-clés Agrovoc : Oryza sativa, Acide folique, Ligase, Hydrolase, Glutamate, Semence, Développement biologique, Physiologie du développement, Activité enzymatique, Expression des gènes, Gène

Mots-clés géographiques Agrovoc : République de Corée

Classification Agris : F62 - Plant physiology - Growth and development
F30 - Plant genetics and breeding

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Anukul Nampeung, University of Nottingham (GBR)
  • Ramos Riza Abilgos, University of Nottingham (GBR)
  • Mehrshahi Payam, University of Michigan (USA)
  • Castelazo Anahi Santoyo, University of Nottingham (GBR)
  • Parker Hélène, University of Nottingham (GBR)
  • Dievart Anne, INRA (FRA) ORCID: 0000-0001-9460-4638
  • Lanau Nadège, CIRAD-BIOS-UMR DAP (FRA)
  • Mieulet Delphine, CIRAD-BIOS-UMR DAP (FRA) ORCID: 0000-0001-6220-0372
  • Tucker Grégory, University of Nottingham (GBR)
  • Guiderdoni Emmanuel, CIRAD-BIOS-UMR DAP (FRA)
  • Barret David A, University of Nottingham (GBR)
  • Bennett Malcolm J., University of Nottingham (GBR)

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