Serodiagnosis of bovine trypanosomosis based on HSP70/BiP inhibition ELISA

Bossard Géraldine, Boulangé Alain, Holzmuller Philippe, Thevenon Sophie, Patrel Delphine, Authié Edith. 2010. Serodiagnosis of bovine trypanosomosis based on HSP70/BiP inhibition ELISA. Veterinary Parasitology, 173 (1-2) : 39-47.

Quartile : Q1, Sujet : VETERINARY SCIENCES / Quartile : Q2, Sujet : PARASITOLOGY

Résumé : Animal trypanosomosis is a serious constraint to livestock productivity in tropical and subtropical countries. The pathogenic trypanosomes in bovidae are Trypanosoma congolense, T. vivax, T. brucei and T. evansi. Current serological tests to detect trypanosome infections are based on the use of whole trypanosome lysates; their potential is limited by antigen instability, lack of reproducibility and lack of test specificity due to the antibody's long persistence after treatment. The development of new tests based on recombinant technology that could be standardized and applied on a large scale at low cost would be very helpful. The major invariant antigen recognized by T. congolense infected cattle belongs to the heat shock protein (HSP) 70 family and is closely related to mammalian Immunoglobulin Binding Protein (BiP). To improve the initial ELISA based on a recombinant fragment of HSP70/BiP, we developed an inhibition ELISA using an anti-BiP monoclonal antibody and a full-length fusion protein expressed in E. coli. Here we report on the development of the test and provide an initial assessment of its performance using sets of sera from experimental infections and from naturally infected cattle maintained in tsetse infested areas of Africa. The HSP70/BIP-based inhibition ELISA shows a good sensitivity in cattle experimentally infected with T. congolense, with an improved sensitivity in secondary infections. One major advantage, particularly for its further application in national laboratories, is that one single set of reagents and one single procedure are sufficient to apply on different mammalian host species infected with different trypanosome species.

Mots-clés Agrovoc : Trypanosoma, Trypanosoma congolense, Trypanosoma brucei, Trypanosoma vivax, immunodiagnostic, zébu, bovin, bétail, Test ELISA, expérimentation, immunoglobuline

Mots-clés géographiques Agrovoc : Afrique au sud du Sahara, Burkina Faso, Kenya

Mots-clés complémentaires : HSP (heat shock-protein)

Classification Agris : L73 - Maladies des animaux
U30 - Méthodes de recherche

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

• Bossard Géraldine, CIRAD-BIOS-UMR TRYPANOSOMES (FRA)
• Boulangé Alain, CIRAD-BIOS-UMR TRYPANOSOMES (MOZ) ORCID: 0000-0002-8505-7465
• Holzmuller Philippe, CIRAD-BIOS-UMR TRYPANOSOMES (FRA) ORCID: 0000-0002-8919-9081
• Thevenon Sophie, CIRAD-BIOS-UMR TRYPANOSOMES (FRA)
• Patrel Delphine, IRD (FRA)
• Authié Edith, AFSSA (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/557546/)

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