Identification of genes involved in the response of banana to crown rot disease

Lassois Ludivine, Frettinger Patrick, De Lapeyre de Bellaire Luc, Lepoivre Philippe, Jijakli M. Haïssam. 2011. Identification of genes involved in the response of banana to crown rot disease. Molecular Plant-Microbe Interactions, 24 (1) : 143-153.

Quartile : Q1, Sujet : PLANT SCIENCES / Quartile : Q1, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY / Quartile : Q1, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Résumé : Variations in banana susceptibility to crown rot disease have been observed but the molecular mechanisms underlying these quantitative host-pathogen relationships are still unknown. This study was designed to compare gene expression between crowns of banana fruit showing a high susceptibility (S+) and crowns showing a low susceptibility (S-) to the disease. Comparisons were performed at two situation times: i) between crowns (S+ and S-) collected 1 h before inoculation and ii) between crowns (S+ and S-) collected 13 days after inoculation. Gene expression comparisons were performed with cDNA-amplified fragment length polymorphism (AFLP) and results were confirmed by realtime reverse-transcription polymerase chain reaction. Among genes identified as differentially expressed between S+ and S- crowns, two were involved in signal transduction, three in proteolytic machinery, two had similarity to pathogenesis- related protein 14, one to a CCR4-associated factor protein, and one to a cellulose synthase. Paradoxically, the overexpression of the cellulose synthase gene was associated with banana showing a high susceptibility in both preand post-inoculation situations. Finally, the cDNA-AFLP identified a gene that seems to be associated with the quantitative banana responses to crown rot disease; this gene encodes a dopamine-?-monooxygenase, which is involved in the catecholamine pathway. To our knowledge, this work is the first to address both pre- and post-infection gene expression with the same host-pathogen combination and distinct susceptibility levels.

Mots-clés Agrovoc : Musa, banane, pourriture, maladie postrécolte, résistance génétique, gène

Mots-clés complémentaires : Pourriture de la couronne

Classification Agris : H20 - Maladies des plantes
F30 - Génétique et amélioration des plantes
J11 - Manutention, transport, stockage et conservation des produits d'origine végétale

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Lassois Ludivine, GxABT (BEL)
• Frettinger Patrick, INRA (FRA)
• De Lapeyre de Bellaire Luc, CIRAD-PERSYST-UPR Systèmes de culture bananes et ananas (FRA) ORCID: 0000-0003-1243-0887
• Lepoivre Philippe, FUSAGx (BEL)
• Jijakli M. Haïssam, GxABT (BEL)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/557624/)

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