Optimized genotyping with microsatellite markers in the fungal banana pathogen Mycosphaerella fijiensis (Mycosphaerellaceae)

Optimized genotyping with microsatellite markers in the fungal banana pathogen Mycosphaerella fijiensis (Mycosphaerellaceae). Robert Stéphanie, Rieux Adrien, Argout Xavier, Carlier Jean, Zapater Marie-Françoise. 2010. American Journal of Botany, 97 (12), e130-e132

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Article de revue ; Article de revue à facteur d'impact

Quartile : Q1, Sujet : PLANT SCIENCES

Résumé : Premise of the study: Large-scale population genetics studies are required to investigate the dispersal processes underlying the emergence of Mycosphaerella fi jiensis , a fungal pathogen of banana. To this end, we have developed an optimized genotyping procedure combining novel microsatellite markers and a modifi ed DNA extraction protocol. Methods and Results: Primers for tetranucleotide loci were designed directly from the recently published genome sequence of M. fijiensis. A total of 19 new polymorphic and easy-to-score markers were developed. Their use was combined with an adapted protocol for total DNA extraction starting from young lesions collected from banana leaves, thus avoiding a pathogen isolation step. Conclusions: The combination of the two technical developments presented here will permit the expansion of genotyping capacity in M. fijiensis, allowing large-scale analysis of samples from various geographic locations. (Résumé d'auteur)

Mots-clés Agrovoc : Musa, Mycosphaerella fijiensis, Génotype, Microsatellite, Marqueur génétique, Maladie des raies noires

Classification Agris : H20 - Maladies des plantes

Axe stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Document en bibliothèque :

Localisation du document : CD_BR18615 [(Bibliothèque de Lavalette)]

Auteurs et affiliations

  • Robert Stéphanie, CIRAD-BIOS-UMR BGPI (FRA)
  • Rieux Adrien, CIRAD-BIOS-UMR BGPI (FRA)
  • Argout Xavier, CIRAD-BIOS-UMR DAP (FRA)
  • Carlier Jean, CIRAD-BIOS-UMR BGPI (FRA)
  • Zapater Marie-Françoise, CIRAD-BIOS-UMR BGPI (FRA)

Source : Cirad - Agritrop

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