Agritrop
Home

Isolation of five new monosomic alien addition lines of Gossypium australe F. Muell in G. hirsutum L. by SSR and GISH analyses

Sarr D., Lacape Jean-Marc, Rodier-Goud Marguerite, Jacquemin Jean-Marie, Benbouza Halima, Toussaint André, Palm Rudy, Ahoton L., Baudoin Jean-Pierre, Mergeai Guy. 2011. Isolation of five new monosomic alien addition lines of Gossypium australe F. Muell in G. hirsutum L. by SSR and GISH analyses. Plant Breeding, 130 (1) : pp. 60-66.

Journal article ; Article de revue à facteur d'impact
[img] Published version - Anglais
Access restricted to CIRAD agents
Use under authorization by the author or CIRAD.
document_558333.pdf

Télécharger (318kB)

Quartile : Q2, Sujet : AGRONOMY / Quartile : Q2, Sujet : PLANT SCIENCES / Quartile : Q3, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Abstract : Gossypium australe F. Muell (2n = 2x = 26) is a wild perennial species possessing agronomic useful traits that would be interesting to introgress into G. hirsutum L. (2n = 4x = 52), the main cultivated cotton species. To isolate monosomic alien addition lines (MAALs) of G. australe in G. hirsutum, the [2(G. hirsutum · G. australe) · G. hirsutum] pentaploid (2n = 5x = 65) was backcrossed as male parent to G. hirsutum. Analysis of 42 BC1 plants and seven alien addition lines, already available, with 150 SSR markers developed from G. hirsutum revealed a cross-species amplification rate of 100% and a polymorphism rate of 56%. Eighty polymorphic SSR markers generated 87 G. australe-specific loci that have been assigned by a hierarchical cluster analysis to 13 linkage groups corresponding to the 13 chromosomes of G. australe. Analysis by SSR markers and genomic in situ hybridization of the self-progeny of disomic alien addition lines, backcross progeny of the pentaploid, allowed the isolation of five new MAALs. (Résumé d'auteur)

Mots-clés Agrovoc : Gossypium, Gossypium hirsutum

Mots-clés géographiques Agrovoc : Belgique

Mots-clés complémentaires : Gossypium australe

Classification Agris : F30 - Plant genetics and breeding

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Sarr D., FUSAGx (BEL)
  • Lacape Jean-Marc, CIRAD-BIOS-UMR DAP (FRA)
  • Rodier-Goud Marguerite, CIRAD-BIOS-UMR DAP (FRA)
  • Jacquemin Jean-Marie, CRA (BEL)
  • Benbouza Halima, FUSAGx (BEL)
  • Toussaint André, FUSAGx (BEL)
  • Palm Rudy, GxABT (BEL)
  • Ahoton L., UNB (BEN)
  • Baudoin Jean-Pierre, FUSAGx (BEL)
  • Mergeai Guy, FUSAGx (BEL)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/558333/)

View Item (staff only) View Item (staff only)

[ Page générée et mise en cache le 2020-11-01 ]