Biological bar code for determining the geographical origin of fruits using 28S rDNA fingerprinting of fungal communities by PCR-DGGE an application to shea tree fruits

El Sheikha Aly, Bouvet Jean-Marc, Montet Didier. 2011. Biological bar code for determining the geographical origin of fruits using 28S rDNA fingerprinting of fungal communities by PCR-DGGE an application to shea tree fruits. Quality Assurance and Safety of Crops and Foods, 3 (1) : 40-47.

Quartile : Q3, Sujet : FOOD SCIENCE & TECHNOLOGY

Résumé : Objectives Shea tree is a multi-purpose tree daily used by rural African communities. Economic importance of Shea tree fruits has been rising and achieving a great success in African, American and European markets. Shea butter is used mainly in chocolate industry, cosmetic or pharmacological products. Traceability is now one of the great concerns of the customers and the lawyers. In view of the difficulties of installing these documentary systems in developing country particularly the countries of sub-Saharan Africa, the new strategies of traceability emerge. Methods Molecular technique using 28S rDNA profiles generated by polymerase chain reaction denaturing gradient gel electrophoresis was used to detect the variation in fungal community structures of Shea tree fruit from Senegal, Mali and Cameroon. Results 28S rDNA profiles were analysed by multivariate analysis, distinct microbial communities were detected. Band profiles of Shea tree fruit fungi from different countries were specific for each location and could be used as a bar code to discriminate the origin of fruits. Conclusion We propose the polymerase chain reaction denaturing gradient gel electrophoresis method as the fingerprinting of Shea tree fruits using 28S rDNA of fungi that provides the fruits with a unique bar code and make it possible to trace back the Shea tree fruit to their original locations.

Mots-clés Agrovoc : Vitellaria paradoxa, flore microbienne, empreinte ADN, provenance, PCR, électrophorèse

Mots-clés géographiques Agrovoc : Mali, Sénégal, Cameroun

Mots-clés complémentaires : Origine géographique

Classification Agris : U30 - Méthodes de recherche
Q04 - Composition des produits alimentaires

Champ stratégique Cirad : Axe 3 (2005-2013) - Alimentation accessible et de qualité

Auteurs et affiliations

• El Sheikha Aly, CIRAD-PERSYST-UMR Qualisud (FRA)
• Bouvet Jean-Marc, CIRAD-BIOS-UPR Génétique forestière (FRA)
• Montet Didier, CIRAD-PERSYST-UMR Qualisud (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/558614/)

Métriques

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