Investigation of pollen competition between wild and cultivated sorghums (Sorghum bicolor (L.) Moench) using simple sequence repeats markers

Muraya Moses, Geiger Hartwig H., De Villiers Santie, Sagnard Fabrice, Kanyenji Ben M., Kiambi Dan, Parzies Heiko K.. 2011. Investigation of pollen competition between wild and cultivated sorghums (Sorghum bicolor (L.) Moench) using simple sequence repeats markers. Euphytica, 178 (3) : pp. 393-401.

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Quartile : Q1, Sujet : HORTICULTURE / Quartile : Q2, Sujet : AGRONOMY / Quartile : Q2, Sujet : PLANT SCIENCES

Abstract : In self-compatible plant species stigmata receive a mixture of self and outcrossed pollen and competition between them is expected to play a major role in determining the pollen-mediated gene flow. The use of male sterile bait plants in field trials to demonstrate the rate of gene flow is questionable due to lack of pollination competition. However, little direct evidence has been published. A field experiment of male sterile and male fertile sorghum pollen recipient bait plants was conducted to evaluate pollen competition between wild and cultivated sorghums and the effects of pollen competition on gene flow assessment. Pollen competition between wild and cultivated sorghums was estimated from two-component pollen mixtures of wild and cultivated sorghum (1:1 ratio) applied to wild, cultivated and male-sterile maternal bait plants. Paternity was determined in the progeny using two diagnostic Simple Sequence Repeat markers. The study found that self pollen has higher seed-siring success. Maternal genotype influences the siring ability of the pollen donor components which significantly deviated from the 1:1 pollen loads. The study showed that published estimates of gene flow derived from studies using male-sterile bait plants seriously overestimate gene flow and that pollen competition may be a significant factor influencing outcrossing rates. The results suggest that the predominant direction of gene flow is from cultivated to wild sorghum, potentially leading to introgression of crop genes into wild sorghum. Pollen competition should be taken into account in gene flow estimation, since presence of self-pollen can account for over half of seed produced irrespective of maternal genotype. (Résumé d'auteur)

Mots-clés Agrovoc : Sorghum bicolor, Compétition végétale, Pollen, Plante de culture, Plante sauvage, Autopollinisation, Pollinisation, Flux de gènes

Mots-clés géographiques Agrovoc : Kenya

Classification Agris : F63 - Plant physiology - Reproduction
F30 - Plant genetics and breeding
F40 - Plant ecology

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Muraya Moses, Institute of Plant Breeding (DEU)
  • Geiger Hartwig H., IRD (FRA)
  • De Villiers Santie, ICRISAT (KEN)
  • Sagnard Fabrice, CIRAD-BIOS-UMR DAP (KEN)
  • Kanyenji Ben M., KARI (KEN)
  • Kiambi Dan, KARI (KEN)
  • Parzies Heiko K., Institute of Plant Breeding (DEU)

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