Thierry Magalie, Lefeuvre Pierre, Hoareau Murielle, Delatte Hélène, Reynaud Bernard, Lett Jean-Michel.
2008. Begomoviruses infecting tobacco in Comoros : Molecular and biological characterization.
In : 14th International BioInformatics Workshop on Virus Evolution and Molecular Epidemiology, Cape Town, South Africa, 1-5 September 2008
Résumé : Begomovirus are wide genera of plant viruses with circular, single-stranded DNA genomes packaged within geminate particles and transmitted by the whitefly Bemisia tabaci. In the South West Indian Ocean (SWIO) islands, the importance of begomovirus diseases is increasing and has spread on many host species since the introduction of a very polyphagous biotype of the insect vector. Within the Regional Program of Plant Protection, an inventory of plant pathogens was organized in the islands of the region, in particular on the Comoros islands where we found tobacco plantations showing severe symptoms of leaf curling. After complete DNA virus genome cloning and sequencing, we identify three begomovirus species: the Tomato leaf curl Mayotte virus, the Tobacco leaf curl Zimbabwe virus and the third that is a new species, temporary named the Tobacco leaf curl Comoros virus. Complete DNA genome sequences were analysed with RDP2 package (Martin et al., 2005) and suggest that the TbLCKMV is a recombinant virus between ToLCYTV and TbLCZV. Sequences were also analysed with PhyML and MrBayes for phylogenetic studies. In order to test the symptomatology (severity) and the differential host range, we produced infectious clones.
Mots-clés Agrovoc : virus des végétaux, Geminiviridae, Nicotiana tabacum, begomovirus
Mots-clés géographiques Agrovoc : Comores
Mots-clés complémentaires : Tobacco leaf curl virus
Classification Agris : H20 - Maladies des plantes
Auteurs et affiliations
- Thierry Magalie, CIRAD-BIOS-UMR PVBMT (REU)
- Lefeuvre Pierre, CIRAD-BIOS-UMR PVBMT (REU)
- Hoareau Murielle, CIRAD-BIOS-UMR PVBMT (REU)
- Delatte Hélène, CIRAD-BIOS-UMR PVBMT (REU)
- Reynaud Bernard, CIRAD-BIOS-UMR PVBMT (REU)
- Lett Jean-Michel, CIRAD-BIOS-UMR PVBMT (REU)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/559522/)
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