Genetic characterization of two traditional leafy vegetables (Sesamum radiatum Thonn. ex Hornem and Ceratotheca sesamoides Endl.) of Benin, using flow cytometry and amplified fragment length polymorphism (AFLP) markers

Adéoti K., Rival Alain, Dansi Alexandre, Santoni Sylvain, Brown Spencer, Beulé Thierry, Nato Aimé, Henry Yves, Vodouhe R.S., Loko L.Y., Sanni Ambaliou. 2011. Genetic characterization of two traditional leafy vegetables (Sesamum radiatum Thonn. ex Hornem and Ceratotheca sesamoides Endl.) of Benin, using flow cytometry and amplified fragment length polymorphism (AFLP) markers. African Journal of Biotechnology, 10 (65) : 14264-14275.

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Résumé : Amplified fragment length polymorphism (AFLP) markers and flow cytometry were applied for the genetic characterization of wild and cultivated accessions of Sesamum radiatum and Cerathoteca sesamoides; two neglected and underutilized species of traditional leafy vegetable consumed in Benin. The average 2C nuclear DNA content per nucleus was found to be 1.99 ± 0.06 and 1.05 ± 0.06 pg for S. radiatum and C. sesamoides, respectively which correspond to estimated genome size of 1946,22 Mpb for S. radiatum and 1026,9 Mpb for C. sesamoides. No variation in DNA content could be detected within accessions from each analysed species. Also, no relation was found between nuclear DNA content, ecogeographical origin and the status (cultivated or wild) of the analyzed accessions. AFLP markers revealed low diversity within the accessions analyzed. Results from the study contributed to a better characterization of S. radiatum and C. sesamoides accessions and will help in defining both genetic resources conservation and breeding strategies.

Mots-clés Agrovoc : Sesamum radiatum, Ceratotheca sesamoides, légume feuille, marqueur génétique, adn, polymorphisme génétique

Mots-clés géographiques Agrovoc : Bénin

Mots-clés complémentaires : AFLP

Classification Agris : F70 - Taxonomie végétale et phytogéographie
F30 - Génétique et amélioration des plantes
U30 - Méthodes de recherche

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

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