Elevated amh gene expression in the brain of male tilapia (Oreochromis niloticus) during testis differentiation

Poonlaphdecha Srisupaph, Pepey Elodie, Huang Sheng-Hui, Canonne Marc, Soler Lucile, Mortaji S., Morand Serge, Pfennig F., Mélard Charles, Baroiller Jean-François, D'Cotta Helena. 2011. Elevated amh gene expression in the brain of male tilapia (Oreochromis niloticus) during testis differentiation. Sexual Development, 5 (1) : 33-47.

Quartile : Q3, Sujet : DEVELOPMENTAL BIOLOGY

Résumé : Anti-müllerian hormone (AMH) is expressed in male embryos and represses development of müllerian ducts during testis differentiation in mammals, birds and reptiles. Amh orthologues have been identified in teleosts despite them lacking müllerian ducts. Previously we found sexually dimorphic aromatase activity in tilapia brains before ovarian differentiation. This prompted us to search for further dimorphisms in tilapia brains during sex differentiation and see whether amh is expressed. We cloned the tilapia amh gene and found that it contains 7 exons but no spliced forms. The putative protein presents highest homologies with Amh proteins of pejerrey and medaka as compared to other Perciformes. We analysed amh expression in adult tissues and found elevated levels in testes, ovary and brain. Amh expression was dimorphic with higher levels in XY male brains at 10-15 dpf, when the gonads were still undifferentiated and gonadal amh was not dimorphic. Male brains had 2.7-fold higher amh expression than gonads. Thereafter, amh levels decreased in the brain while they were up-regulated in differentiating testes. Our study indicates that amh is transcribed in male brains already at 10 dpf, suggesting that sexual differentiation may be occurring earlier in tilapia brain than in gonads.

Mots-clés Agrovoc : Oreochromis niloticus, Tilapia, dimorphisme sexuel, différenciation sexuelle, détermination du sexe, hormone

Classification Agris : L53 - Physiologie animale - Reproduction
L10 - Génétique et amélioration des animaux
M12 - Production de l'aquaculture

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Poonlaphdecha Srisupaph, CIRAD-PERSYST-UPR Aquaculture (FRA)
• Pepey Elodie, CIRAD-PERSYST-UMR INTREPID (FRA)
• Huang Sheng-Hui
• Canonne Marc, CIRAD-PERSYST-UMR INTREPID (FRA)
• Soler Lucile, CIRAD-PERSYST-UMR INTREPID (FRA)
• Mortaji S.
• Morand Serge, CIRAD-ES-UPR AGIRs (FRA) ORCID: 0000-0003-3986-7659
• Pfennig F., Institute of Zoology (DEU)
• Mélard Charles, Centre de Recherches et de Formation en Aquaculture (BEL)
• Baroiller Jean-François, CIRAD-PERSYST-UMR INTREPID (FRA) ORCID: 0000-0002-0992-1725
• D'Cotta Helena, CIRAD-PERSYST-UMR INTREPID (FRA) ORCID: 0000-0003-2739-3476

Source : Cirad - Agritrop (https://agritrop.cirad.fr/562984/)

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