Molecular diagnosis by PCR-DHPLC technique of wood-decay fungi in historical buildings in Italy

Zaremski Alba, Palanti Sabrina, Mannucci Massimo, Gastonguay Louis, Le Floch Gaetan. 2011. Molecular diagnosis by PCR-DHPLC technique of wood-decay fungi in historical buildings in Italy. Pro Ligno, 7 (4) : pp. 92-97.

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Titre roumain; moldave : Diagnostic molecular prin tehnica PCR-DHPLC a ciupercilor ce degradeaza lemnul in cladirile istorice din Italia

Abstract : Wood inhabiting fungi cause real problems in the preservation of wooden surfaces and are responsible for the deterioration of cultural heritage. The identification of fungi based on morphological characteristics is still a topical issue. Nevertheless, they are limited for characterization and identification on an intraspecific level and even sometimes on an interspecific level. It is not always evident and thus many fungi remain unnamed or confused. The objective of this study was to circumvent these limitations by using a new molecular approach allowing fungal detection and identification in historic buildings in Italy. Fungal colonization was assessed by using PCR amplification and amplicons separation by Denaturing High Performance Liquid Chromatography. Due to its high sensitivity, the PCR-DHPLC technique was optimised to profile fungal communities in wood decay as well as ubiquitous contaminants. (Résumé d'auteur)

Mots-clés Agrovoc : Bois de charpente, PCR, Diagnostic, Champignon, Carie du bois, Préservation du bois

Mots-clés géographiques Agrovoc : Italie

Classification Agris : N01 - Agricultural engineering
J12 - Handling, transport, storage and protection of forest products
U30 - Research methods
K50 - Processing of forest products

Champ stratégique Cirad : Hors axes (2005-2013)

Auteurs et affiliations

  • Zaremski Alba, CIRAD-BIOS-UMR AGAP (FRA)
  • Palanti Sabrina, IVALSA (ITA)
  • Mannucci Massimo
  • Gastonguay Louis, IREQ (CAN)
  • Le Floch Gaetan, ESMISAB (FRA)

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Source : Cirad - Agritrop (

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