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Use of the Capripoxvirus homologue of Vaccinia virus 30kDa RNA polymerase subunit (RPO30) gene as a novel diagnostic and genotyping target : Development of a classical PCR method to differentiate Goat poxvirus from Sheep poxvirus

Lamien Charles Euloge, Le Goff Christian, Silber Roland, Wallace David B., Gulyaz Vely, Tuppurainen Eeva, Madani Hafsa, Caufour Philippe, Adam Tajelser, El Harrak Mehdi, Luckins Antony George, Albina Emmanuel, Diallo Adama. 2011. Use of the Capripoxvirus homologue of Vaccinia virus 30kDa RNA polymerase subunit (RPO30) gene as a novel diagnostic and genotyping target : Development of a classical PCR method to differentiate Goat poxvirus from Sheep poxvirus. Veterinary Microbiology, 149 (1-2) : pp. 30-39.

Journal article ; Article de revue à facteur d'impact
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Quartile : Outlier, Sujet : VETERINARY SCIENCES / Quartile : Q2, Sujet : MICROBIOLOGY

Abstract : Sheep poxvirus (SPPV), Goat poxvirus (GTPV) and Lumpy skin disease virus (LSDV) are Capripoxviruses (CaPVs) responsible for causing severe poxvirus disease in sheep, goats and cattle, respectively. Serological differentiation of CaPVs is not possible and strain identification has relied on the implicitly accepted hypothesis that the viruses show well defined host specificity. However, it is now known that cross infections can occur and authentication of identity based on the host animal species from which the strain was first isolated, is not valid and should be replaced with molecular techniques to allow unequivocal strain differentiation. To identify a diagnostic target for strain genotyping, the CaPV homologue of the Vaccinia virus E4L gene which encodes the 30 kDa DNA-dependant RNA polymerase subunit, RPO30 was analyzed. Forty-six isolates from different hosts and geographical origins were included. Most CaPVs fit into one of the three different groups according to their host origins: the SPPV, the GTPV and the LSDV group. A unique 21-nucleotide deletion was found in all SPPV isolates which was exploited to develop a RPO30-based classical PCR test to differentiate SPPV from GTPV that will allow rapid differential diagnosis of disease during CaPV outbreaks in small ruminants. (Résumé d'auteur)

Mots-clés Agrovoc : Capripoxvirus, Virose, Virologie, Immunologie, Identification, Méthode, Test biologique, Mouton, Chèvre, Maladie de la peau, Diagnostic, Génotype

Mots-clés complémentaires : Variole ovine, Clavelée

Classification Agris : L73 - Animal diseases
L50 - Animal physiology and biochemistry
U30 - Research methods

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

  • Lamien Charles Euloge, AIEA (AUT)
  • Le Goff Christian, CIRAD-BIOS-UMR CMAEE (FRA)
  • Silber Roland, Institute for Veterinary Disease Control (AUT)
  • Wallace David B., ARC (ZAF)
  • Gulyaz Vely, Pendik Veterinary Control and Research Institute (TUR)
  • Tuppurainen Eeva, Institute of Animal Health (GBR)
  • Madani Hafsa, Institut national de la médecine vétérinaire (DZA)
  • Caufour Philippe, CIRAD-BIOS-UMR CMAEE (FRA)
  • Adam Tajelser, Central Veterinary Research Laboratory (SDN)
  • El Harrak Mehdi, Biopharma (MAR)
  • Luckins Antony George, AIEA (AUT)
  • Albina Emmanuel, CIRAD-BIOS-UMR CMAEE (FRA)
  • Diallo Adama, CIRAD-BIOS-UMR CMAEE (AUT)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/563360/)

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