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Nineteen polymorphic microsatellite markers from two african Vanilla species: across-species transferability and diversity in a wild population of V. humblotii from Mayotte

Gigant Rodolphe, Brugel Anaïs, De Bruyn Alexandre, Risterucci Ange-Marie, Guiot Valérie, Viscardi G., Humeau Laurence, Grisoni Michel, Besse Pascale. 2012. Nineteen polymorphic microsatellite markers from two african Vanilla species: across-species transferability and diversity in a wild population of V. humblotii from Mayotte. Conservation Genetics Resources, 4 (1) : pp. 121-125.

Journal article ; Article de revue à facteur d'impact
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Quartile : Q3, Sujet : BIODIVERSITY CONSERVATION / Quartile : Q4, Sujet : GENETICS & HEREDITY

Abstract : There is a serious lack of information on the genetic diversity and population dynamics of the 110 tropical Vanilla species, although these are keys elements to adjust conservation strategies. Nineteen polymorphic microsatellite markers were developed from two African leafless Vanilla species V. roscheri and V. humblotii to use in population genetic studies. A transferability analysis of these markers on seven Vanilla species from various geographical origins was conducted. Nine microsatellites were polymorphic in a population of 22 individuals of V. humblotii from Mayotte (Comoros Archipelago). These markers had two to eight alleles per locus and observed heterozygosity ranged from 0.23 to 0.64. Amplification parameters were calibrated to facilitate multiplexing and rapid multi-loci genotyping. (Résumé d'auteur)

Mots-clés Agrovoc : Vanilla, Microsatellite, Dynamique des populations, Conservation des ressources, Variation génétique

Mots-clés géographiques Agrovoc : Réunion, Comores

Mots-clés complémentaires : Vanilla humblotii, Vanilla roscheri

Classification Agris : F30 - Plant genetics and breeding
F70 - Plant taxonomy and geography

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Gigant Rodolphe, CIRAD-BIOS-UMR PVBMT (REU)
  • Brugel Anaïs
  • De Bruyn Alexandre, CIRAD-BIOS-UMR PVBMT (REU)
  • Risterucci Ange-Marie, CIRAD-BIOS-UMR AGAP (FRA)
  • Guiot Valérie, CBNM Antenne de Mayotte (MYT)
  • Viscardi G., CBNM Antenne de Mayotte (MYT)
  • Humeau Laurence, Université de la Réunion (REU)
  • Grisoni Michel, CIRAD-BIOS-UMR PVBMT (REU)
  • Besse Pascale, Université de la Réunion (REU)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/563439/)

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