The half-size ABC transporters STR1 and STR2 are indispensable for mycorrhizal arbuscule formation in rice

Gutjahr Caroline, Radovanovic Dragica, Geoffroy Jessika, Zhang Quan, Siegler Heike, Chiapello Marco, Casieri Leonardo, An Kyungsook, An Gynheung, Guiderdoni Emmanuel, Kumar Chellian Santhosh, Sundaresan Venkatesan, Harrison Maria J., Paszkowski Uta. 2012. The half-size ABC transporters STR1 and STR2 are indispensable for mycorrhizal arbuscule formation in rice. Plant Journal, 69 (5) : pp. 906-920.

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Quartile : Outlier, Sujet : PLANT SCIENCES

Abstract : The central structure of the symbiotic association between plants and arbuscular mycorrhizal (AM) fungi is the fungal arbuscule that delivers minerals to the plant. Our earlier transcriptome analyses identified two half-size ABCG transporters that displayed enhanced mRNA levels in mycorrhizal roots. We now show specific transcript accumulation in arbusculated cells of both genes during symbiosis. Presently, arbuscule-relevant factors from monocotyledons have not been reported. Mutation of either of the Oryza sativa (rice) ABCG transporters blocked arbuscule growth of different AM fungi at a small and stunted stage, recapitulating the phenotype of Medicago truncatula stunted arbuscule 1 and 2 (str1 and str2) mutants that are deficient in homologous ABCG genes. This phenotypic resemblance and phylogenetic analysis suggest functional conservation of STR1 and STR2 across the angiosperms. Malnutrition of the fungus underlying limited arbuscular growth was excluded by the absence of complementation of the str1 phenotype by wild-type nurse plants. Furthermore, plant AM signaling was found to be intact, as arbuscule-induced marker transcript accumulation was not affected in str1 mutants. Strigolactones have previously been hypothesized to operate as intracellular hyphal branching signals and possible substrates of STR1 and STR2. However, full arbuscule development in the strigolactone biosynthesis mutants d10 and d17 suggested strigolactones to be unlikely substrates of STR1/STR2. Interestingly, rice STR1 is associated with a cis-natural antisense transcript (antiSTR1). Analogous to STR1 and STR2, at the root cortex level, the antiSTR1 transcript is specifically detected in arbusculated cells, suggesting unexpected modes of STR1 regulation in rice. (Résumé d'auteur)

Mots-clés Agrovoc : Oryza sativa, Gigaspora, Glomus intraradices, Symbiose, Mycorhizé à vésicule et arbuscule, Transport des substances nutritives, Phénotype, Medicago truncatula, Physiologie végétale, Physiologie de la nutrition, Gène, ARN, ADN, Phylogénie

Mots-clés complémentaires : Gigaspora rosea, Oryza sativa japonica

Classification Agris : F62 - Plant physiology - Growth and development
F61 - Plant physiology - Nutrition
F30 - Plant genetics and breeding

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Gutjahr Caroline, UNIL (CHE)
  • Radovanovic Dragica, UNIL (CHE)
  • Geoffroy Jessika, UNIL (CHE)
  • Zhang Quan, Cornell University (USA)
  • Siegler Heike, UNIL (CHE)
  • Chiapello Marco, UNIL (CHE)
  • Casieri Leonardo, UNIL (CHE)
  • An Kyungsook, Kyung Hee University (KOR)
  • An Gynheung, Kyung Hee University (KOR)
  • Guiderdoni Emmanuel, CIRAD-BIOS-UMR AGAP (FRA)
  • Kumar Chellian Santhosh, UC (USA)
  • Sundaresan Venkatesan, UC (USA)
  • Harrison Maria J., Cornell University (USA)
  • Paszkowski Uta, UNIL (CHE)

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