Proteomic plasticity of two Eucalyptus genotypes under contrasted water regimes in the field

Bedon Franck, Villar Emilie, Vincent Delphine, Dupuy Jean-William, Lomenech Anne Marie, Mabialangoma André, Chaumeil Philippe, Barré Aurélien, Plomion Christophe, Gion Jean-Marc. 2012. Proteomic plasticity of two Eucalyptus genotypes under contrasted water regimes in the field. Plant, Cell and Environment, 35 (4) : pp. 790-805.

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Quartile : Q1, Sujet : PLANT SCIENCES

Abstract : Water deficit affects tree growth and limits wood production. In an attempt to identify the molecular triggers of adaptation mechanisms to water deficit in Eucalyptus, we investigated protein expression patterns of two ecophysiologically contrasted Eucalyptus genotypes. They were grown in the field in either natural conditions or irrigated for 7 weeks during the dry season in the Republic of Congo. At the phenotypic level, genotype (G), treatment (T) and/or G ¥ T interaction effects were observed for aboveand below-ground biomass-related traits. At the molecular level, changes in protein abundance were recorded in leaves (acidic pH 4-7, and basic pH 7-11, proteomes) and stems (acidic proteome) using two-dimensional gel electrophoresis (2-DE). One third of the detected protein spots displayed significantG,T and/orG ¥ T effects, and 158 of them were identified by tandem mass spectrometry (LC-MS/MS) analysis. Thus, several proteins whose molecular plasticity was genetically controlled (i.e. G ¥ T effect) were revealed, highlighting adaptive mechanisms to water deficit specific to each genotype, namely cell wall modification, cell detoxification and osmoregulation. Transcript abundances corresponding to G ¥ T proteins were also investigated by quantitative RT-PCR. These proteins represent relevant targets to improve drought resistance in this ecologically and economically important forest tree genus. (Résumé d'auteur)

Mots-clés Agrovoc : Eucalyptus, Eucalyptus grandis, Eucalyptus urophylla, Adaptabilité, Électrophorèse, Expression des gènes, Génotype, Stress dû à la sécheresse, Déficit hydrique du sol

Mots-clés géographiques Agrovoc : Congo

Mots-clés complémentaires : Eucalyptus alba, Protéomique

Classification Agris : F30 - Plant genetics and breeding
K10 - Forestry production
P33 - Soil chemistry and physics
F62 - Plant physiology - Growth and development

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Bedon Franck, INRA (FRA)
  • Villar Emilie, CIRAD-BIOS-UMR AGAP (FRA)
  • Vincent Delphine, INRA (FRA)
  • Dupuy Jean-William, Université de Bordeaux (FRA)
  • Lomenech Anne Marie, Université de Bordeaux (FRA)
  • Mabialangoma André, CRDPI (COG)
  • Chaumeil Philippe, INRA (FRA)
  • Barré Aurélien, Université de Bordeaux II (FRA)
  • Plomion Christophe, INRA (FRA)
  • Gion Jean-Marc, CIRAD-BIOS-UMR AGAP (FRA)

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