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MultiLocus Sequence Analysis- and Amplified Fragment Length Polymorphism-based characterization of xanthomonads associated with bacterial spot of tomato and pepper and their relatedness to Xanthomonas species

Hamza Abdou Azali, Robène-Soustrade Isabelle, Jouen Emmanuel, Lefeuvre Pierre, Chiroleu Frédéric, Fisher-Le Saux Marion, Gagnevin Lionel, Pruvost Olivier. 2012. MultiLocus Sequence Analysis- and Amplified Fragment Length Polymorphism-based characterization of xanthomonads associated with bacterial spot of tomato and pepper and their relatedness to Xanthomonas species. Systematic and Applied Microbiology, 35 (3) : pp. 183-190.

Journal article ; Article de revue à facteur d'impact
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Quartile : Q2, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY / Quartile : Q2, Sujet : MICROBIOLOGY

Abstract : MultiLocus Sequence Analysis (MLSA) and Amplified Fragment Length Polymorphism (AFLP) were used to measure the genetic relatedness of a comprehensive collection of xanthomonads pathogenic to solaneous hosts to Xanthomonas species. The MLSA scheme was based on partial sequences of four housekeeping genes (atpD, dnaK, efp and gyrB). Globally, MLSA data unambiguously identified strains causing bacterial spot of tomato and pepper at the species level and was consistent with AFLP data. Genetic distances derived from both techniques showed a close relatedness of (i) X. euvesicatoria, X. perforans and X. alfalfae and (ii) X. gardneri and X. cynarae. Maximum likelihood tree topologies derived from each gene portion and the concatenated data set for species in the X. campestris 16S rRNA core (i.e. the species cluster comprising all strains causing bacterial spot of tomato and pepper) were not congruent, consistent with the detection of several putative recombination events in our data sets by several recombination search algorithms. One recombinant region in atpD was identified in most strains of X. euvesicatoria including the type strain. (Résumé d'auteur)

Mots-clés Agrovoc : Xanthomonas, Brassicaceae, Polymorphisme génétique, Phylogénie, Solanaceae

Mots-clés géographiques Agrovoc : Réunion

Mots-clés complémentaires : AFLP

Classification Agris : H20 - Plant diseases

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Hamza Abdou Azali, Université de la Réunion (REU)
  • Robène-Soustrade Isabelle, CIRAD-BIOS-UMR PVBMT (REU)
  • Jouen Emmanuel, Université de la Réunion (REU)
  • Lefeuvre Pierre, CIRAD-BIOS-UMR PVBMT (REU)
  • Chiroleu Frédéric, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0002-4874-5357
  • Fisher-Le Saux Marion, INRA (FRA)
  • Gagnevin Lionel, CIRAD-BIOS-UMR PVBMT (REU)
  • Pruvost Olivier, CIRAD-BIOS-UMR PVBMT (REU)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/563612/)

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