Xu Jianlong, Zheng H.J., Liu L., Pan Z.C., Prior Philippe.
2011. Research on pathogenic variation of Ralstonia solanacearum strain Po82 and its genome.
In : Abstract book : The fifth international bacterial wilt symposium. OCRI CAAS
Résumé : R. solanacearum, as a species complex, can be divided into four phylotypes on the basis of phylotype subclassification system proposed by Fegan and Prior. In our phylogenetic research, Po82, a potato bv1 strain from Mexico, was resolved into phylotype II sequevar 4, in a group with phylotype II/4NPB and banana Moko disease-causing strains. It was identified as Moko strains' sequevar 4 (MLG25) according to Mmx-PCR. Additionally, pathogenicity test revealed that Po82 possess pathogenic traits of both NPB and Moko strains thereby proving that it was a pathogenicity variation strain. The genome sequence of the Po82 genome was determined by using a massively parallel pyrosequencing technology (Roche 454 GS FLX) .The genome size of Po82 is 5.34 Mb, with an average GC content of 66.7%. Compared to seven sequenced R. solanacearum strains, 465 strain-unique genes were identified within the genome of strain Po82, most of which encode hypothetical and conserved hypothetical proteins. Furthermore, 48 putative genomic islands were predicted within Po82 genome.
Classification Agris : H20 - Maladies des plantes
Auteurs et affiliations
- Xu Jianlong
- Zheng H.J.
- Liu L.
- Pan Z.C.
- Prior Philippe, CIRAD-BIOS-UMR PVBMT (REU)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/563770/)
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