Identification of a new phospholipase D in Carica papaya latex

Abdelkafi Slim, Abousalham Abdelkarim, Fendri Imen, Ogata Hiroshi Y., Barouh Nathalie, Fouquet Benjamin, Scheirlinckx Frantz, Villeneuve Pierre, Carrière Frédéric. 2012. Identification of a new phospholipase D in Carica papaya latex. Gene, 499 (2) : 243-249.

https://doi.org/10.1016/j.gene.2012.03.033

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Quartile : Q3, Sujet : GENETICS & HEREDITY

Résumé : Phospholipase D (PLD) is a lipolytic enzyme involved in signal transduction, vesicle trafficking and membrane metabolism. It catalyzes the hydrolysis and transphosphatidylation of glycerophospholipids at the terminal phosphodiester bond. The presence of a PLD in the latex of Carica papaya (CpPLD1) was demonstrated by transphosphatidylation of phosphatidylcholine (PtdCho) in the presence of 2% ethanol. Although the protein could not be purified to homogeneity due to its presence in high molecular mass aggregates, a protein band was separated by SDS-PAGE after SDS/chloroform-methanol/TCA-acetone extraction of the latex insoluble fraction. This material was digested with trypsin and the amino acid sequences of the tryptic peptides were determined by micro-LC/ESI/MS/MS. These sequences were used to identify a partial cDNA (723 bp) from expressed sequence tags (ESTs) of C. papaya. Based upon EST sequences, a full-length gene was identified in the genome of C. papaya, with an open reading frame of 2424 bp encoding a protein of 808 amino acid residues, with a theoretical molecular mass of 92.05 kDa. From sequence analysis, CpPLD1 was identified as a PLD belonging to the plant phosphatidylcholine phosphatidohydrolase family.

Mots-clés Agrovoc : Carica papaya, latex, phospholipase, spectrométrie de masse

Mots-clés géographiques Agrovoc : Congo

Classification Agris : F30 - Génétique et amélioration des plantes
F60 - Physiologie et biochimie végétale

Champ stratégique Cirad : Hors axes (2005-2013)

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