A simple protocol for cryopreservation of zygotic embryos of ten accessions of coconut (Cocos nucifera L.)

N'Nan Oulo, Borges M., Konan Konan Jean Louis, Hocher Valérie, Verdeil Jean-Luc, Tregear James, N'Guetta Assanvo Simon-Pierre, Engelmann Florent, Malaurie Bernard. 2012. A simple protocol for cryopreservation of zygotic embryos of ten accessions of coconut (Cocos nucifera L.). In Vitro Cellular and Developmental Biology. Plant, 48 (2) : pp. 160-166.

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Quartile : Q3, Sujet : PLANT SCIENCES / Quartile : Q4, Sujet : CELL BIOLOGY / Quartile : Q4, Sujet : DEVELOPMENTAL BIOLOGY

Abstract : A simple and efficient cryopreservation protocol for coconut zygotic embryos has been developed. Embryos were inoculated in Petri dishes on medium containing 3.2 M glucose, which were placed in hermetically closed containers containing 80 or 160 g silica gel for 48 or 24 h. Moisture content of embryos at the end of this treatment varied between 0.25 and 0.65 g g?1 DW, depending on the accession. Embryos were then transferred for cryopreservation by rapid immersion of cryotubes in liquid nitrogen. After rapid re-warming, embryos were transferred to culture medium containing Eeuwens mineral elements for germination. This protocol was applied to ten accessions representative of coconut genetic diversity, with germination percentages of cryopreserved embryos between 13.7% and 74.7%. (Résumé d'auteur)

Mots-clés Agrovoc : Cocos nucifera, Culture d'embryon, Conservation du matériel génétique, Cryoconservation

Mots-clés géographiques Agrovoc : Côte d'Ivoire

Classification Agris : F62 - Plant physiology - Growth and development
F01 - Crop husbandry

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • N'Nan Oulo, IRD (FRA)
  • Borges M., Universidad Central de Gramma (CUB)
  • Konan Konan Jean Louis, CNRA (CIV)
  • Hocher Valérie, IRD (FRA)
  • Verdeil Jean-Luc, CIRAD-BIOS-UMR AGAP (FRA)
  • Tregear James, IRD (FRA)
  • N'Guetta Assanvo Simon-Pierre, Université de Cocody (CIV)
  • Engelmann Florent, IRD (FRA)
  • Malaurie Bernard, Bioversity International (ITA)

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