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Enzymatic production of monoacylglycerols (MAG) and diacylglycerols (DAG) from fish oil in a solvent-free system

Feltes Maria Manuela Camino, Villeneuve Pierre, Baréa Bruno, Barouh Nathalie, De Oliveira J. Vladimir, De Oliveira Débora, Ninow Jorge Luiz. 2012. Enzymatic production of monoacylglycerols (MAG) and diacylglycerols (DAG) from fish oil in a solvent-free system. Journal of the American Oil Chemists' Society, 89 (6) : pp. 1057-1065.

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Quartile : Q2, Sujet : CHEMISTRY, APPLIED / Quartile : Q2, Sujet : FOOD SCIENCE & TECHNOLOGY

Abstract : Mono- (MAG) and diacylglycerols (DAG) are of nutritional interest. MAG and DAG containing eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids were produced in a solvent-free system via glycerolysis of menhaden oil catalyzed by Novozym 435. The effect of the molar ratio of glycerol to oil, enzyme concentration, and reaction temperature on MAG and DAG production was assessed. The optimal temperature was in the range of 55-70 _C for production of both acylglycerols. The increase in the substrates molar ratio led to a decrease in MAG and DAG content. The enzyme concentration was fixed at the lowest level evaluated (5%, by weight of substrates). High content of MAG (25% by weight) and DAG (41% by weight) containing, respectively, 12.46% EPA and 11.16% DHA, and 14.57% EPA and 13.70% DHA, were produced after 24 h at 70 _C, with 5% of lipase (by weight of substrate) and a glycerol-to-oil molar ratio of 1:1. For this reaction, a molar triacylglycerol (TAG) conversion of about 60% was achieved at equilibrium (10 h). (Résumé d'auteur)

Mots-clés Agrovoc : Huile de poisson, Acylglycérole, Biosynthèse, Activité enzymatique, Estérase, Lipase

Classification Agris : Q02 - Food processing and preservation

Champ stratégique Cirad : Axe 3 (2005-2013) - Alimentation accessible et de qualité

Auteurs et affiliations

  • Feltes Maria Manuela Camino, UFSC (BRA)
  • Villeneuve Pierre, CIRAD-PERSYST-UMR IATE (FRA) ORCID: 0000-0003-1685-1494
  • Baréa Bruno, CIRAD-PERSYST-UMR IATE (FRA)
  • Barouh Nathalie, CIRAD-PERSYST-UMR IATE (FRA)
  • De Oliveira J. Vladimir, Universidade Regional Integrada do Alto Uruguai e das Missões (BRA)
  • De Oliveira Débora, Universidade Regional Integrada do Alto Uruguai e das Missões (BRA)
  • Ninow Jorge Luiz, UFSC (BRA)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/564258/)

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