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Marker-free transgenic durum wheat cv. Karim expressing the AlSAP gene exhibits a high level of tolerance to salinity and dehydration stresses

Ben-Saad Rania, Ben-Ramdhan Walid, Zouari Nabil, Azaza Jalel, Mieulet Delphine, Guiderdoni Emmanuel, Ellouz Radhouane, Hassairi Afif. 2012. Marker-free transgenic durum wheat cv. Karim expressing the AlSAP gene exhibits a high level of tolerance to salinity and dehydration stresses. Molecular Breeding, 30 (1) : pp. 521-533.

Journal article ; Article de revue à facteur d'impact
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Quartile : Q1, Sujet : HORTICULTURE / Quartile : Q1, Sujet : AGRONOMY / Quartile : Q1, Sujet : PLANT SCIENCES / Quartile : Q2, Sujet : GENETICS & HEREDITY

Abstract : We have recently isolated the AlSAP (stress-associated protein) gene from the halophyte grass Aeluropus littoralis and demonstrated that AlSAP expression improves tolerance to continuous salt and drought stresses in transgenic tobacco. To extend these findings to an important crop, we generated marker-free transgenic durum wheat plants of the commercial cv. Karim expressing the AlSAP gene. The integration and expression of AlSAP in T3 homozygous plants were ascertained by Southern, Northern and Western blotting respectively. AlSAP wheat lines exhibited improved germination rates and biomass production under severe salinity and osmotic stress conditions. Following a long-term salt or drought stress greenhouse trial, AlSAP lines produced normally filled grains whereas wild-type (WT) plants either died at the vegetative stage under salt stress or showed markedly reduced grain filling under drought stress. Measurements of the RWC (relative water content) and endogenous Na? and K? levels in leaves of AlSAP plants, showed a lower water loss rate and a higher Na? accumulation in senescent-basal leaves, respectively, compared to those of WT plants. Taken together, these results extend to cereals the high potential of the AlSAP gene for engineering effective drought and salt tolerance. (Résumé d'auteur)

Mots-clés Agrovoc : Triticum durum, Plante transgénique, Transfert de gène, Expression des gènes, Tolérance au sel, Résistance à la sécheresse, Résistance génétique, Stress abiotique, Génie génétique, Marqueur génétique

Mots-clés géographiques Agrovoc : Tunisie, France, Arabie Saoudite

Mots-clés complémentaires : Aeluropus littoralis

Classification Agris : F30 - Plant genetics and breeding
H50 - Miscellaneous plant disorders
F62 - Plant physiology - Growth and development

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Ben-Saad Rania, Centre of Biotechnology of Sfax (TUN)
  • Ben-Ramdhan Walid, Centre of Biotechnology of Sfax (TUN)
  • Zouari Nabil, Centre of Biotechnology of Sfax (TUN)
  • Azaza Jalel, Centre of Biotechnology of Sfax (TUN)
  • Mieulet Delphine, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-6220-0372
  • Guiderdoni Emmanuel, CIRAD-BIOS-UMR AGAP (FRA)
  • Ellouz Radhouane, Centre of Biotechnology of Sfax (TUN)
  • Hassairi Afif, Centre de biotechnologie (TUN)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/564333/)

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