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Identification of novel microRNAs in Hevea brasiliensis and computational prediction of their targets

Gebelin Virginie, Argout Xavier, Engchuan Worrawat, Pitollat Bertrand, Duan Cuifang, Montoro Pascal, Leclercq Julie. 2012. Identification of novel microRNAs in Hevea brasiliensis and computational prediction of their targets. BMC Plant Biology, 12 (12), 18 p.

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Quartile : Q1, Sujet : PLANT SCIENCES

Résumé : Background Plants respond to external stimuli through fine regulation of gene expression partially ensured by small RNAs. Of these, microRNAs (miRNAs) play a crucial role. They negatively regulate gene expression by targeting the cleavage or translational inhibition of target messenger RNAs (mRNAs). In Hevea brasiliensis, environmental and harvesting stresses are known to affect natural rubber production. This study set out to identify abiotic stress-related miRNAs in Hevea using next-generation sequencing and bioinformatic analysis. Results Deep sequencing of small RNAs was carried out on plantlets subjected to severe abiotic stress using the Solexa technique. By combining the LeARN pipeline, data from the Plant microRNA database (PMRD) and Hevea EST sequences, we identified 48 conserved miRNA families already characterized in other plant species, and 10 putatively novel miRNA families. The results showed the most abundant size for miRNAs to be 24 nucleotides, except for seven families. Several MIR genes produced both 20-22 nucleotides and 23-27 nucleotides. The two miRNA class sizes were detected for both conserved and putative novel miRNA families, suggesting their functional duality. The EST databases were scanned with conserved and novel miRNA sequences. MiRNA targets were computationally predicted and analysed. The predicted targets involved in "responses to stimuli" and to "antioxidant" and "transcription activities" are presented. Conclusions Deep sequencing of small RNAs combined with transcriptomic data is a powerful tool for identifying conserved and novel miRNAs when the complete genome is not yet available. Our study provided additional information for evolutionary studies and revealed potentially specific regulation of the control of redox status in Hevea. (Résumé d'auteur)

Mots-clés Agrovoc : Hevea brasiliensis, Séquence nucléotidique, Identification, Expression des gènes, Bioinformatique, ARN, Oxydation, Latex, Perte à la récolte, Régulation physiologique

Mots-clés complémentaires : Séquencage

Classification Agris : F30 - Génétique et amélioration des plantes
U10 - Méthodes mathématiques et statistiques
F60 - Physiologie et biochimie végétales
H50 - Troubles divers des plantes

Axe stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Gebelin Virginie, CIRAD-BIOS-UMR AGAP (FRA)
  • Argout Xavier, CIRAD-BIOS-UMR AGAP (FRA)
  • Engchuan Worrawat, KMUTT (THA)
  • Pitollat Bertrand, CIRAD-BIOS-UMR AGAP (FRA)
  • Duan Cuifang, CATAS (CHN)
  • Montoro Pascal, CIRAD-BIOS-UMR AGAP (FRA)
  • Leclercq Julie, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-8110-3762

Source : Cirad - Agritrop

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