Biodiversity of Mimosa pudica rhizobial symbionts (Cupriavidus taiwanensis, Rhizobium mesoamericanum) in New Caledonia and their adaptation to heavy metal-rich soils

Klonowska Agnieszka, Chaintreuil Clémence, Tisseyre Pierre, Miche Lucie, Melkonian Rémy, Ducousso Marc, Laguerre Gisèle, Brunel Brigitte, Moulin Lionel. 2012. Biodiversity of Mimosa pudica rhizobial symbionts (Cupriavidus taiwanensis, Rhizobium mesoamericanum) in New Caledonia and their adaptation to heavy metal-rich soils. FEMS Microbiology, Ecology, 81 (3) : 618-635.

Quartile : Q2, Sujet : MICROBIOLOGY

Résumé : Rhizobia are soil bacteria able to develop a nitrogen-fixing symbiosis with legumes. They are taxonomically spread among the alpha and beta subclasses of the Proteobacteria. Mimosa pudica, a tropical invasive weed, has been found to have an affinity for beta-rhizobia, including species within the Burkholderia and Cupriavidus genera. In this study, we describe the diversity of M. pudica symbionts in the island of New Caledonia, which is characterized by soils with high heavy metal content, especially of Ni. By using a plant-trapping approach on four soils, we isolated 96 strains, the great majority of which belonged to the species Cupriavidus taiwanensis (16S rRNA and recA gene phylogenies). A few Rhizobium strains in the newly described species Rhizobium mesoamericanum were also isolated. The housekeeping and nod gene phylogenies supported the hypothesis of the arrival of the C. taiwanensis and R. mesoamericanum strains together with their host at the time of the introduction of M. pudica in New Caledonia (NC) for its use as a fodder. The C. taiwanensis strains exhibited various tolerances to Ni, Zn and Cr, suggesting their adaptation to the specific environments in NC. Specific metal tolerance marker genes were found in the genomes of these symbionts, and their origin was investigated by phylogenetic analyses.

Mots-clés Agrovoc : Mimosa pudica, symbiose, Rhizobium, phylogénie, biodiversité, sol pollué, nickel, métal lourd, bioremédiation, Burkholderiaceae

Mots-clés géographiques Agrovoc : Nouvelle-Calédonie, France

Mots-clés complémentaires : Cupriavidus taiwanensis

Classification Agris : F62 - Physiologie végétale - Croissance et développement
F70 - Taxonomie végétale et phytogéographie
F40 - Écologie végétale
P36 - Érosion, conservation et récupération des sols

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Klonowska Agnieszka, INRA (FRA)
• Chaintreuil Clémence, IRD (FRA)
• Tisseyre Pierre, IRD (FRA)
• Miche Lucie, IRD (FRA)
• Melkonian Rémy, IRD (FRA)
• Ducousso Marc, CIRAD-BIOS-UMR LSTM (FRA)
• Laguerre Gisèle, INRA (FRA)
• Brunel Brigitte, INRA (FRA)
• Moulin Lionel, IRD (FRA) ORCID: 0000-0001-9068-6912

Source : Cirad - Agritrop (https://agritrop.cirad.fr/565371/)

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