Genome sequencing of Xanthomonas axonopodis pv. phaseoli CFBP4834-R reveals that flagellar motility is not a general feature of xanthomonads.

Darrasse Armelle, Carrère Sébastien, Barbe Valérie, Bourreau Tristan, Bernal Adriana, Bonneau Sophie, Brin Christelle, Cociancich Stéphane, Durand Karine, Fouteau Stéphanie, Gagnevin Lionel, Gouzy Jérôme, Guérin Fabien, Guy Endrick, Indiana Arnaud, Koebnik Ralf, Lauber Emmanuelle, Munoz Alejandra, Noel Laurent D., Pieretti Isabelle, Poussier Stéphane, Pruvost Olivier, Robène-Soustrade Isabelle, Rott Philippe, Royer Monique, Szurek Boris, Van Sluys Marie-Anne, Verdier Valérie, Vernière Christian, Arlat Mathieu, Manceau Charles, Jacques Marie Agnès. 2012. Genome sequencing of Xanthomonas axonopodis pv. phaseoli CFBP4834-R reveals that flagellar motility is not a general feature of xanthomonads.. In : 4th Xanthomonas genomics conference, Angers, France, july 9-12, 2012 : Book of abstracts. Manceau Charles (ed.), Jacques Marie Agnès (ed.), Arlat Mathieu (ed.), Lauber Emmanuelle (ed.), Noel Laurent D. (ed.), Gagnevin Lionel (ed.), Pruvost Olivier (ed.), Rott Philippe (ed.), Royer Monique (ed.), Koebnik Ralf (ed.), Verdier Valérie (ed.). INRA, INRA-LIPM, CIRAD-BIOS-UMR PVBMT, IRD. Paris : INRA, Résumé, p. 13. Xanthomonas genomics conference 2012. 4, Angers, France, 9 July 2012/12 July 2012.

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Abstract : Xanthomonads are plant-associated bacteria that establish neutral, commensal or pathogenic relationships with plants. The list of common characteristics shared by all members of the genus Xanthomonas is now well established based on the entire genome sequences that are currently available and that represent various species, numerous pathovars of X. axonopodis (sensu Vauterin et al., 2000), X. oryzae and X. campestris, and many strains within some pathovars. These ?-proteobacteria are motile by a single polar flagellum. Motility is an important feature involved in biofilm formation, plant colonization and hence considered as a pathogenicity factor. X. axonopodis pv. phaseoli var. fuscans (Xapf) is one of the causal agents of common bacterial blight of bean and 4834-R is a highly aggressive strain of this pathogen that was isolated from a seed-borne epidemic in France in 1998. We obtained a high quality assembled sequence of the genome of this strain with 454-Solexa and 2X Sanger sequencing. Housekeeping functions are conserved in this genome that shares core characteristics with genomes of other xanthomonads: the six secretion systems which have been described so far in Gram negative bacteria are all present, as well as their ubiquitous substrates or effectors and a rather usual number of mobile elements. Elements devoted to the adaptation to the environment constitute an important part of the genome with a chemotaxis island and dispersed MCPs, numerous two-component systems, and numerous TonB dependent transporters. Furthermore, numerous multidrug efflux systems and functions dedicated to biofilm formation that confer resistance to stresses are also present. An intriguing feature revealed by genome analysis is a long deletion of 35 genes (33 kbp) involved in flagellar biosynthesis. This deletion is replaced by an insertion sequence called ISXapf2. Genes such as flgB to flgL and fliC to fleQ which are involved in the flagellar structure (rod, P- and L-ring, hook, cap and filament) are absent in the genome of strain 4834-R that is not motile. Primers were designed to detect this deletion by PCR in a collection of more than 300 strains representing different species and pathovars of Xanthomonas, and less than 5% of the tested xanthomonads strains were found nonmotile because of a deletion in the flagellum gene cluster. We observed that half of the Xapf strains isolated from the same epidemic than strain 4834-R was non-motile and that this ratio was conserved in the strains colonizing the next bean seed generation. Isolation of such variants in a natural epidemic reveals that either flagellar motility is not a key function for fitness or that some complementation occurs within the bacterial population. (Résumé d'auteur)

Classification Agris : H20 - Plant diseases

Auteurs et affiliations

  • Darrasse Armelle, INRA (FRA)
  • Carrère Sébastien, INRA (FRA)
  • Barbe Valérie, Centre national de séquençage (FRA)
  • Bourreau Tristan, INRA (FRA)
  • Bernal Adriana, Universidad de los Andes (COL)
  • Bonneau Sophie, Agrocampus Ouest (FRA)
  • Brin Christelle, INRA (FRA)
  • Cociancich Stéphane, CIRAD-BIOS-UMR BGPI (FRA)
  • Durand Karine, INRA (FRA)
  • Fouteau Stéphanie, CEA (FRA)
  • Gagnevin Lionel, CIRAD-BIOS-UMR PVBMT (REU)
  • Gouzy Jérôme, INRA (FRA)
  • Guérin Fabien, Université de la Réunion (REU)
  • Guy Endrick, INRA (FRA)
  • Indiana Arnaud, Agrocampus Ouest (FRA)
  • Koebnik Ralf, IRD (FRA)
  • Lauber Emmanuelle, INRA (FRA)
  • Munoz Alejandra, Universidad de los Andes (COL)
  • Noel Laurent D., INRA (FRA)
  • Pieretti Isabelle, CIRAD-BIOS-UMR BGPI (FRA)
  • Poussier Stéphane, INRA (FRA)
  • Pruvost Olivier, CIRAD-BIOS-UMR PVBMT (REU)
  • Robène-Soustrade Isabelle, CIRAD-BIOS-UMR PVBMT (REU)
  • Rott Philippe, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-6085-6159
  • Royer Monique, CIRAD-BIOS-UMR BGPI (FRA)
  • Szurek Boris, IRD (FRA)
  • Van Sluys Marie-Anne, Universidade de São Paulo (BRA)
  • Verdier Valérie, IRD (FRA)
  • Vernière Christian, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0002-2312-2073
  • Arlat Mathieu, Université Toulouse III Paul Sabatier (FRA)
  • Manceau Charles, INRA (FRA)
  • Jacques Marie Agnès, INRA (FRA)

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