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Genomic insights into strategies used by Xanthomonas albilineans with its reduced artillery to spread within sugarcane xylem vessels

Pieretti Isabelle, Royer Monique, Barbe Valérie, Carrère Sébastien, Koebnik Ralf, Couloux Arnaud, Darrasse Armelle, Gouzy Jérôme, Jacques Marie Agnès, Lauber Emmanuelle, Manceau Charles, Mangenot Sophie, Poussier Stéphane, Segurens Béatrice, Szurek Boris, Verdier Valérie, Arlat Mathieu, Gabriel Dean W., Rott Philippe, Cociancich Stéphane. 2012. Genomic insights into strategies used by Xanthomonas albilineans with its reduced artillery to spread within sugarcane xylem vessels. BMC Genomics, 13 (658), 23 p.

Journal article ; Article de revue à facteur d'impact Revue en libre accès total
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Quartile : Q1, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY / Quartile : Q1, Sujet : GENETICS & HEREDITY

Abstract : Background Xanthomonas albilineans causes leaf scald, a lethal disease of sugarcane. X. albilineans exhibits distinctive pathogenic mechanisms, ecology and taxonomy compared to other species of Xanthomonas. For example, this species produces a potent DNA gyrase inhibitor called albicidin that is largely responsible for inducing disease symptoms; its habitat is limited to xylem; and the species exhibits large variability. A first manuscript on the complete genome sequence of the highly pathogenic X. albilineans strain GPE PC73 focused exclusively on distinctive genomic features shared with Xylella fastidiosa-another xylem-limited Xanthomonadaceae. The present manuscript on the same genome sequence aims to describe all other pathogenicity-related genomic features of X. albilineans, and to compare, using suppression subtractive hybridization (SSH), genomic features of two strains differing in pathogenicity. Results Comparative genomic analyses showed that most of the known pathogenicity factors from other Xanthomonas species are conserved in X. albilineans, with the notable absence of two major determinants of the "artillery" of other plant pathogenic species of Xanthomonas: the xanthan gum biosynthesis gene cluster, and the type III secretion system Hrp (hypersensitive response and pathogenicity). Genomic features specific to X. albilineans that may contribute to specific adaptation of this pathogen to sugarcane xylem vessels were also revealed. SSH experiments led to the identification of 20 genes common to three highly pathogenic strains but missing in a less pathogenic strain. These 20 genes, which include four ABC transporter genes, a methyl-accepting chemotaxis protein gene and an oxidoreductase gene, could play a key role in pathogenicity. With the exception of hypothetical proteins revealed by our comparative genomic analyses and SSH experiments, no genes potentially involved in any offensive or counter-defensive mechanism specific to X. albilineans were identified, supposing that X. albilineans has a reduced artillery compared to other pathogenic Xanthomonas species. Particular attention has therefore been given to genomic features specific to X. albilineans making it more capable of evading sugarcane surveillance systems or resisting sugarcane defense systems. Conclusions This study confirms that X. albilineans is a highly distinctive species within the genus Xanthomonas, and opens new perpectives towards a greater understanding of the pathogenicity of this destructive sugarcane pathogen. (Résumé d'auteur)

Mots-clés Agrovoc : Xanthomonas albilineans, Saccharum officinarum, Pouvoir pathogène, Génome, Séquence nucléotidique, Phylogénie, Relation hôte pathogène, Xylème

Mots-clés géographiques Agrovoc : Sri Lanka, Papouasie-Nouvelle-Guinée, Brésil, Burkina Faso, Taïwan, Floride, Réunion, Guadeloupe, Martinique

Classification Agris : H20 - Plant diseases

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Pieretti Isabelle, CIRAD-BIOS-UMR BGPI (FRA)
  • Royer Monique, CIRAD-BIOS-UMR BGPI (FRA)
  • Barbe Valérie, Centre national de séquençage (FRA)
  • Carrère Sébastien, INRA (FRA)
  • Koebnik Ralf, IRD (FRA)
  • Couloux Arnaud, Centre national de séquençage (FRA)
  • Darrasse Armelle, INRA (FRA)
  • Gouzy Jérôme, INRA (FRA)
  • Jacques Marie Agnès, INRA (FRA)
  • Lauber Emmanuelle, INRA (FRA)
  • Manceau Charles, INRA (FRA)
  • Mangenot Sophie, Centre national de séquençage (FRA)
  • Poussier Stéphane, INRA (FRA)
  • Segurens Béatrice, Centre national de séquençage (FRA)
  • Szurek Boris, IRD (FRA)
  • Verdier Valérie, IRD (FRA)
  • Arlat Mathieu, Université Toulouse III Paul Sabatier (FRA)
  • Gabriel Dean W., University of Florida (USA)
  • Rott Philippe, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-6085-6159
  • Cociancich Stéphane, CIRAD-BIOS-UMR BGPI (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/566855/)

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